Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance | Sciact - CRIS-system of Boreskov Institute of Catalysis

Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance Full article

Journal

Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827

Output data

Year: 2022, Volume: 38, Number: 8, Pages: 2550–2560 Pages count : 11 DOI: 10.1021/acs.langmuir.1c03096

Tags

Nutrition, Vesicles, Deformation, Surface plasmon resonance, Organic compounds

Authors

Norling Karin ¹ , Sjöberg Mattias ¹ , Bally Marta ^2,3 , Zhdanov Vladimir P. ^1,4 , Parveen Nagma ¹ , Höök Fredrik ¹

Affiliations

1	Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, 412 96 Gothenburg, Sweden
2	Department of Clinical Microbiology, Umeå University, 901 85 Umeå, Sweden; Wallenberg Centre for Molecular Medicine, Umeå University, 901 85 Umeå, Sweden;
3	Wallenberg Centre for Molecular Medicine, Umeå University, 901 85 Umeå, Sweden
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Funding (3)

1	Stiftelsen för strategisk forskning	IRC15-0065
2	Knut and Alice Wallenberg Foundation	2019-0577
3	Swedish Research Council	2018-04900

The mechanical properties of biological nanoparticles play a crucial role in their interaction with the cellular membrane, in particular for cellular uptake. This has significant implications for the design of pharmaceutical carrier particles. In this context, liposomes have become increasingly popular, among other reasons due to their customizability and easily varied physicochemical properties. With currently available methods, it is, however, not trivial to characterize the mechanical properties of nanoscopic liposomes especially with respect to the level of deformation induced upon their ligand–receptor-mediated interaction with laterally fluid cellular membranes. Here, we utilize the sensitivity of dual-wavelength surface plasmon resonance to probe the size and shape of bound liposomes (∼100 nm in diameter) as a means to quantify receptor-induced deformation during their interaction with a supported cell membrane mimic. By comparing biotinylated liposomes in gel and fluid phases, we demonstrate that fluid-phase liposomes are more prone to deformation than their gel-phase counterparts upon binding to the cell membrane mimic and that, as expected, the degree of deformation depends on the number of ligand–receptor pairs that are engaged in the multivalent binding.

Norling K. , Sjöberg M. , Bally M. , Zhdanov V.P. , Parveen N. , Höök F.
Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance
Langmuir. 2022. V.38. N8. P.2550–2560. DOI: 10.1021/acs.langmuir.1c03096 WOS Scopus РИНЦ AN PMID OpenAlex

Submitted:	Nov 18, 2021
Accepted:	Jan 17, 2022
Published online:	Feb 14, 2022
Published print:	Mar 1, 2022

Web of science:	WOS:000766043400016
Scopus:	2-s2.0-85125117622
Elibrary:	48184086
Chemical Abstracts:	2022:334532
PMID:	35156833
OpenAlex:	W4213057379

DB	Citing
Scopus	8
Web of science	8
Elibrary	4
OpenAlex	7