Investigation of the Chromatographic Behaviour of Metabolites from Blood Plasma by HPLC-MS/MS Using a Monolithic Column with a Sorbent Based on 1-Vinyl-1,2,4-triazole Full article
| Journal |
Chemistry for Sustainable Development
ISSN: 1817-1818 |
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| Output data | Year: 2022, Volume: 30, Number: 6, Pages: 571-578 Pages count : 8 DOI: 10.15372/csd2022419 | ||||||
| Tags | 1-vinyl-1,2,4-triazole, monolithic columns, high performance liquid chromatography, mass spectrometry, chromatography of hydrophilic interactions, reversed-phase chromatography, metabolomics | ||||||
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| Affiliations |
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Funding (1)
| 1 | Russian Science Foundation | 22-23-01068 (121122500037-0) |
Abstract:
The chromatographic behavior of a number of human blood plasma metabolites in HPLC-MS/MS analysis using a monolithic chromatographic column based on 1-vinyl-1,2,4-triazole was studied. Acetonitrile and a buffer based on ammonium carbonate and ammonia (pH 9.8) were used as the mobile phase components. The experiment was carried out in two chromatographic modes: reversed-phase and hydrophilic. The metabolites selected for analysis allow for an unambiguous demonstration of the behaviour of both polar molecules and hydrophobic or amphiphilic lipid molecules in each of the chromatographic modes used. It was discovered that the change of chromatographic mode was accompanied by an inversion of the order of elution of metabolite groups having differences in chemical structure and hydrophilicity. It was shown that the monolithic column based on 1-vinyl-1,2,4-triazole can be used to screen metabolites in both reversed-phase and hydrophilic chromatographic modes. The use of two chromatography modes in metabolome screening is mutually complementary and can help to increase the coverage of metabolites from different metabolic pathways.
Cite:
Basov N.V.
, Rogachev A.D.
, Sotnikova Y.S.
, Patrushev Y.V.
, Pokrovsky A.G.
Investigation of the Chromatographic Behaviour of Metabolites from Blood Plasma by HPLC-MS/MS Using a Monolithic Column with a Sorbent Based on 1-Vinyl-1,2,4-triazole
Chemistry for Sustainable Development. 2022. V.30. N6. P.571-578. DOI: 10.15372/csd2022419 WOS РИНЦ OpenAlex
Investigation of the Chromatographic Behaviour of Metabolites from Blood Plasma by HPLC-MS/MS Using a Monolithic Column with a Sorbent Based on 1-Vinyl-1,2,4-triazole
Chemistry for Sustainable Development. 2022. V.30. N6. P.571-578. DOI: 10.15372/csd2022419 WOS РИНЦ OpenAlex
Original:
Басов Н.В.
, Рогачев А.Д.
, Сотникова Ю.С.
, Патрушев Ю.В.
, Покровский А.Г.
Исследование хроматографического поведения метаболитов из плазмы крови методом ВЭЖХ-МС/МС с использованием монолитной колонки с сорбентом на основе 1-винил-1,2,4-триазола
Химия в интересах устойчивого развития. 2022. Т.30. №6. С.591-598. DOI: 10.15372/KhUR2022419 РИНЦ AN OpenAlex
Исследование хроматографического поведения метаболитов из плазмы крови методом ВЭЖХ-МС/МС с использованием монолитной колонки с сорбентом на основе 1-винил-1,2,4-триазола
Химия в интересах устойчивого развития. 2022. Т.30. №6. С.591-598. DOI: 10.15372/KhUR2022419 РИНЦ AN OpenAlex
Identifiers:
| Web of science: | WOS:000898674300001 |
| Elibrary: | 49923485 |
| OpenAlex: | W4313067897 |