Unraveling How Cholesterol Affects Multivalency-Induced Membrane Deformation of Sub-100 nm Lipid Vesicles | Sciact - CRIS-система Института катализа

Unraveling How Cholesterol Affects Multivalency-Induced Membrane Deformation of Sub-100 nm Lipid Vesicles Научная публикация

Журнал

Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827

Вых. Данные

Год: 2022, Том: 38, Номер: 51, Страницы: 15950-15959 Страниц : 10 DOI: 10.1021/acs.langmuir.2c02252

Ключевые слова

water permeability; delivery; nanoparticles; model

Авторы

Park Hyeonjin ^1,2 , Sut Tun Naw ¹ , Yoon Bo Kyeong ³ , Zhdanov Vladimir P. ^4,5 , Cho Nam-Joon ² , Jackman Joshua A. ¹

Организации

1	School of Chemical Engineering and Translational Nanobioscience Research Center, Sungkyunkwan University, Suwon 16419, Republic of Korea
2	School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore
3	School of Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Republic of Korea
4	Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg 41296, Sweden
5	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Информация о финансировании (5)

1	National Research Foundation of Korea	2020R1C1C1004385
2	National Research Foundation of Korea	2021R1A4A1032782
3	National Research Foundation of Korea	2020K1A3A1A39112724
4	Ministry of Health and Welfare	HI19C1328
5	Sungkyunkwan University

Cholesterol plays a critical role in modulating the lipid membrane properties of biological and biomimetic systems and recent attention has focused on its role in the functions of sub-100 nm lipid vesicles and lipid nanoparticles. These functions often rely on multivalent ligand–receptor interactions involving membrane attachment and dynamic shape transformations while the extent to which cholesterol can influence such interaction processes is largely unknown. To address this question, herein, we investigated the attachment of sub-100 nm lipid vesicles containing varying cholesterol fractions (0–45 mol %) to membrane-mimicking supported lipid bilayer (SLB) platforms. Biotinylated lipids and streptavidin proteins were used as model ligands and receptors, respectively, while the localized surface plasmon resonance sensing technique was employed to track vesicle attachment kinetics in combination with analytical modeling of vesicle shape changes. Across various conditions mimicking low and high multivalency, our findings revealed that cholesterol-containing vesicles could bind to receptor-functionalized membranes but underwent appreciably less multivalency-induced shape deformation than vesicles without cholesterol, which can be explained by a cholesterol-mediated increase in membrane bending rigidity. Interestingly, the extent of vesicle deformation that occurred in response to increasingly strong multivalent interactions was less pronounced for vesicles with greater cholesterol fraction. The latter trend was rationalized by taking into account the strong dependence of the membrane bending energy on the area of the vesicle–SLB contact region and such insights can aid the engineering of membrane-enveloped nanoparticles with tailored biophysical properties.

Park H. , Sut T.N. , Yoon B.K. , Zhdanov V.P. , Cho N-J. , Jackman J.A.
Unraveling How Cholesterol Affects Multivalency-Induced Membrane Deformation of Sub-100 nm Lipid Vesicles
Langmuir. 2022. V.38. N51. P.15950-15959. DOI: 10.1021/acs.langmuir.2c02252 WOS Scopus РИНЦ CAPlusCA PMID OpenAlex

Поступила в редакцию:	19 авг. 2022 г.
Принята к публикации:	14 дек. 2022 г.
Опубликована online:	14 дек. 2022 г.
Опубликована в печати:	27 дек. 2022 г.

Web of science:	WOS:000898894200001
Scopus:	2-s2.0-85144302383
РИНЦ:	50420622
Chemical Abstracts:	2022:3092634
Chemical Abstracts (print):	181:139132
PMID (PubMed):	36515977
OpenAlex:	W4311473774

БД	Цитирований
Web of science	4
Scopus	3
РИНЦ	1
OpenAlex	3