pH-Modulated Nanoarchitectonics for Enhancement of Multivalency-Induced Vesicle Shape Deformation at Receptor-Presenting Lipid Membrane Interfaces | Sciact - CRIS-система Института катализа

pH-Modulated Nanoarchitectonics for Enhancement of Multivalency-Induced Vesicle Shape Deformation at Receptor-Presenting Lipid Membrane Interfaces Научная публикация

Журнал

Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827

Вых. Данные

Год: 2023, Том: 39, Номер: 23, Страницы: 8297–8305 Страниц : 9 DOI: 10.1021/acs.langmuir.3c00777

Ключевые слова

Deformation, Lipids, Membranes, Surface plasmon resonance, Vesicles

Авторы

Park Hyeonjin ^1,2 , Sut Tun Naw ^1,2 , Ferhan Abdul Rahim ³ , Yoon Bo Kyeong ⁴ , Zhdanov Vladimir P. ^5,6 , Cho Nam-Joon ³ , Jackman Joshua A. ^1,2

Организации

1	School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea
2	Translational Nanobioscience Research Center, Sungkyunkwan University, Suwon 16419, Republic of Korea
3	School of Materials Science and Engineering, Nanyang Technological University, 637553, Singapore
4	School of Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Republic of Korea
5	Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg 41296, Sweden
6	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Информация о финансировании (5)

1	National Research Foundation of Korea	2020R1C1C1004385
2	National Research Foundation of Korea	2021R1A4A1032782
3	National Research Foundation of Korea	2020K1A3A1A39112724
4	Ministry of Health and Welfare	HI19C1328
5	Sungkyunkwan University

Multivalent ligand–receptor interactions between receptor-presenting lipid membranes and ligand-modified biological and biomimetic nanoparticles influence cellular entry and fusion processes. Environmental pH changes can drive these membrane-related interactions by affecting membrane nanomechanical properties. Quantitatively, however, the corresponding effects on high-curvature, sub-100 nm lipid vesicles are scarcely understood, especially in the multivalent binding context. Herein, we employed the label-free localized surface plasmon resonance (LSPR) sensing technique to track the multivalent attachment kinetics, shape deformation, and surface coverage of biotin ligand-functionalized, zwitterionic lipid vesicles with different ligand densities on a streptavidin receptor-coated supported lipid bilayer under varying pH conditions (4.5, 6, 7.5). Our results demonstrate that more extensive multivalent interactions caused greater vesicle shape deformation across the tested pH conditions, which affected vesicle surface packing as well. Notably, there were also pH-specific differences, i.e., a higher degree of vesicle shape deformation was triggered at a lower multivalent binding energy in pH 4.5 than in pH 6 and 7.5 conditions. These findings support that the nanomechanical properties of high-curvature lipid membranes, especially the membrane bending energy and the corresponding responsiveness to multivalent binding interactions, are sensitive to solution pH, and indicate that multivalency-induced vesicle shape deformation occurs slightly more readily in acidic pH conditions relevant to biological environments.

Park H. , Sut T.N. , Ferhan A.R. , Yoon B.K. , Zhdanov V.P. , Cho N-J. , Jackman J.A.
pH-Modulated Nanoarchitectonics for Enhancement of Multivalency-Induced Vesicle Shape Deformation at Receptor-Presenting Lipid Membrane Interfaces
Langmuir. 2023. V.39. N23. P.8297–8305. DOI: 10.1021/acs.langmuir.3c00777 WOS Scopus РИНЦ CAPlusCA PMID OpenAlex

Поступила в редакцию:	22 мар. 2023 г.
Принята к публикации:	18 мая 2023 г.
Опубликована online:	2 июн. 2023 г.
Опубликована в печати:	13 июн. 2023 г.

Web of science:	WOS:001010226200001
Scopus:	2-s2.0-85163264583
РИНЦ:	54050065
Chemical Abstracts:	2023:1130065
Chemical Abstracts (print):	183:202228
PMID (PubMed):	37267480
OpenAlex:	W4379114196

БД	Цитирований
РИНЦ	1
Web of science	2
OpenAlex	1
Scopus	2