Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection | Sciact - CRIS-system of Boreskov Institute of Catalysis

Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection Full article

Journal

Polymers
, E-ISSN: 2073-4360

Output data

Year: 2023, Volume: 15, Number: 15, Article number : 3202, Pages count : 20 DOI: 10.3390/polym15153202

Tags

electrospun scaffold; DNA delivery; plasmid DNA; nanoparticle; DNA release; cell transfection; manipulating cellular behaviors

Authors

Chernonosova Vera ¹ , Khlebnikova Marianna ¹ , Popova Victoriya ¹ , Starostina Ekaterina ² , Kiseleva Elena ³ , Chelobanov Boris ¹ , Kvon Ren ⁴ , Dmitrienko Elena ¹ , Laktionov Pavel ¹

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia
2	State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia
3	Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia
4	Boreskov Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia

Funding (1)

Ministry of Science and Higher Education of the Russian Federation

075-00404-21-00 (121031300042-1)

Biomaterial-mediated, spatially localized gene delivery is important for the development of cell-populated scaffolds used in tissue engineering. Cells adhering to or penetrating into such a scaffold are to be transfected with a preloaded gene that induces the production of secreted proteins or cell reprogramming. In the present study, we produced silica nanoparticles-associated pDNA and electrospun scaffolds loaded with such nanoparticles, and studied the release of pDNA from scaffolds and cell-to-scaffold interactions in terms of cell viability and pDNA transfection efficacy. The pDNA-coated nanoparticles were characterized with dynamic light scattering and transmission electron microscopy. Particle sizes ranging from 56 to 78 nm were indicative of their potential for cell transfection. The scaffolds were characterized using scanning electron microscopy, X-ray photoelectron spectroscopy, stress-loading tests and interaction with HEK293T cells. It was found that the properties of materials and the pDNA released vary, depending on the scaffold’s composition. The scaffolds loaded with pDNA-nanoparticles do not have a pronounced cytotoxic effect, and can be recommended for cell transfection. It was found that (pDNA-NPs) + PEI9-loaded scaffold demonstrates good potential for cell transfection. Thus, electrospun scaffolds suitable for the transfection of inhabiting cells are eligible for use in tissue engineering.

Chernonosova V. , Khlebnikova M. , Popova V. , Starostina E. , Kiseleva E. , Chelobanov B. , Kvon R. , Dmitrienko E. , Laktionov P.
Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection
Polymers. 2023. V.15. N15. 3202 :1-20. DOI: 10.3390/polym15153202 WOS Scopus РИНЦ AN PMID OpenAlex

Submitted:	Jun 16, 2023
Accepted:	Jul 26, 2023
Published online:	Jul 27, 2023
Published print:	Aug 1, 2023

Web of science:	WOS:001046322400001
Scopus:	2-s2.0-85167796551
Elibrary:	54361431
Chemical Abstracts:	2023:1717223
PMID:	37571096
OpenAlex:	W4385348825

DB	Citing
OpenAlex	3
Web of science	3
Elibrary	2
Scopus	3