Stability Study of the Antiviral Agent Camphecene in Dried Blood Spots at Different Temperatures | Sciact - CRIS-система Института катализа

Stability Study of the Antiviral Agent Camphecene in Dried Blood Spots at Different Temperatures Научная публикация

Журнал

Drug Testing and Analysis
ISSN: 1942-7611

Вых. Данные

Год: 2021, Том: 13, Номер: 10, Страницы: 1797-1802 Страниц : 6 DOI: 10.1002/dta.3148

Ключевые слова

antiviral agents, camphecene, dried blood spots, sample preparation, stability

Авторы

Okhina Alina A. ^1,2 , Rogachev Artem D. ^1,2 , Yarovaya Olga I. ^1,2 , Pokrovsky Andrey G. ² , Salakhutdinov Nariman F. ^1,2

Организации

1	Department of Medicinal chemistry, N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Novosibirsk, Russia
2	V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, Novosibirsk, Russia

In this study, an optimized procedure of sample preparation for quantitative determination of the antiviral agent camphecene in dried rat blood spots was developed. It has been shown that when using methanol containing 0.1% HCOOH as an extractant, the recovery of the substance increases in comparison with the previously developed method. In addition to this, there is no need to dilute the obtained solutions with water for the analysis of the sample by high-performance liquid chromatography (HPLC) on a column with a reversed-phase sorbent. By using the developed method, the stability of samples of dried rat blood spots containing camphecene in different concentrations at different temperatures was studied. It was found that while the samples were stored at room temperature, apparently, desorption of the substance occurs leading to a loss of more than 15% of its initial amount after 5–10 days. Lowering the temperature increases the stability of samples and their storage at −70°C is possible for 4 weeks.

Okhina A.A. , Rogachev A.D. , Yarovaya O.I. , Pokrovsky A.G. , Salakhutdinov N.F.
Stability Study of the Antiviral Agent Camphecene in Dried Blood Spots at Different Temperatures
Drug Testing and Analysis. 2021. V.13. N10. P.1797-1802. DOI: 10.1002/dta.3148 WOS Scopus CAPlusCA PMID OpenAlex

Поступила в редакцию:	31 июл. 2021 г.
Принята к публикации:	11 авг. 2021 г.
Опубликована в печати:	1 окт. 2021 г.

Web of science:	WOS:000692776200001
Scopus:	2-s2.0-85113459736
Chemical Abstracts:	2021:1898490
Chemical Abstracts (print):	176:360074
PMID (PubMed):	34448348
OpenAlex:	W3193835941

БД	Цитирований
OpenAlex	4
Web of science	5
Scopus	5