Gene Networks for Use in Metabolomic Data Analysis of Blood Plasma from Patients with Postoperative Delirium | Sciact - CRIS-система Института катализа

Gene Networks for Use in Metabolomic Data Analysis of Blood Plasma from Patients with Postoperative Delirium Научная публикация

Журнал

Вавиловский журнал генетики и селекции
ISSN: 2500-0462 , E-ISSN: 2500-3259

Вых. Данные

Год: 2023, Том: 27, Номер: 7, Страницы: 768-775 Страниц : 8 DOI: 10.18699/vjgb-23-89

Ключевые слова

LC-MS/MS; metabolomics; lipidomics; postoperative delirium; cardiac surgery; biomarkers; sphingolipids; gene networks; ANDSystem; ВЭЖХ-МС/МС, метаболомика, липидомика, послеоперационный делирий, кардиохирургия, биомаркеры, сфинголипиды, генные сети, ANDSystem

Авторы

Ivanisenko V.A. ^1,2,7 , Basov N.V. ^2,3 , Makarova A.A. ¹ , Venzel A.S. ^1,7 , Rogachev A.D. ^2,3 , Demenkov P.S. ^1,2,7 , Ivanisenko T.V. ^1,2,7 , Kleshchev M.A. ¹ , Gaisler E.V. ^2,3 , Moroz G.B. ⁴ , Plesko V.V. ⁴ , Sotnikova Y.S. ^2,3,5 , Patrushev Y.V. ^2,5 , Lomivorotov V.V. ^4,6 , Kolchanov N.A. ^1,2 , Pokrovsky A.G. ²

Организации

1	Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук, Новосибирск, Россия
2	Новосибирский национальный исследовательский государственный университет, Новосибирск, Россия
3	Новосибирский институт органической химии им. Н.Н. Ворожцова Сибирского отделения Российской академии наук, Новосибирск, Россия
4	Национальный медицинский исследовательский центр им. академика Е.Н. Мешалкина Министерства здравоохранения Российской Федерации, Новосибирск, Россия
5	Федеральный исследовательский центр «Институт катализа им. Г.К. Борескова Сибирского отделения Российской академии наук», Новосибирск, Россия
6	Медицинский центр им. Милтона Херши, Херши, Пенсильвания, США
7	Курчатовский геномный центр ИЦиГ СО РАН, Новосибирск, Россия

Информация о финансировании (1)

Российский научный фонд

22-23-01068 (121122500037-0)

Postoperative delirium (POD) is considered one of the most severe complications, resulting in impaired cognitive function, extended hospitalization, and higher treatment costs. The challenge of early POD diagnosis becomes particularly significant in cardiac surgery cases, as the incidence of this complication exceeds 50 % in certain patient categories. While it is known that neuroinflammation, neurotransmitter imbalances, disruptions in neuroendocrine regulation, and interneuronal connections contribute significantly to the development of POD, the molecular, genetic mechanisms of POD in cardiac surgery patients, along with potential metabolomic diagnostic markers, remain inadequately understood. In this study, blood plasma was collected from a group of patients over 65 years old after cardiac surgery involving artificial circulation. The collected samples were analyzed for sphingomyelin content and quantity using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) methods. The analysis revealed four significantly different sphingomyelin contents in patients with POD compared to those who did not develop POD (control group). Employing gene network reconstruction, we perceived a set of 82 regulatory enzymes affiliated with the genetic coordination of the sphingolipid metabolism pathway. Within this set, 47 are assumed to be regulators of gene expression, governing the transcription of enzymes pivotal to the metabolic cascade. Complementing this, an additional assembly of 35 regulators are considered to be regulators of activity, degradation, and translocation dynamics of enzymes integral to the aforementioned pathway. Analysis of the overrepresentation of diseases with which these regulatory proteins are associated showed that the regulators can be categorized into two groups, associated with cardiovascular pathologies (CVP) and neuropsychiatric diseases (NPD), respectively. The regulators associated with CVP are expectedly related to the effects on myocardial tissue during surgery. It is hypothesized that dysfunction of NPD-associated regulators may specifically account for the development of POD after cardiac surgery. Thus, the identified regulatory genes may provide a basis for planning further experiments, in order to study disorders at the level of expression of these genes, as well as impaired function of proteins encoded by them in patients with POD. The identified significant sphingolipids can be considered as potential markers of POD.

Ivanisenko V.A. , Basov N.V. , Makarova A.A. , Venzel A.S. , Rogachev A.D. , Demenkov P.S. , Ivanisenko T.V. , Kleshchev M.A. , Gaisler E.V. , Moroz G.B. , Plesko V.V. , Sotnikova Y.S. , Patrushev Y.V. , Lomivorotov V.V. , Kolchanov N.A. , Pokrovsky A.G.
Gene Networks for Use in Metabolomic Data Analysis of Blood Plasma from Patients with Postoperative Delirium
Вавиловский журнал генетики и селекции. 2023. V.27. N7. P.768-775. DOI: 10.18699/vjgb-23-89 WOS Scopus РИНЦ PMID OpenAlex

Поступила в редакцию:	21 июл. 2023 г.
Принята к публикации:	24 авг. 2023 г.

Web of science:	WOS:001140097800006
Scopus:	2-s2.0-85181519269
РИНЦ:	5669725
PMID (PubMed):	38223851
OpenAlex:	W4389624098

БД	Цитирований
OpenAlex	8
Scopus	7
Web of science	6