The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in E. coli | Sciact - CRIS-система Института катализа

The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in E. coli Научная публикация

Журнал

BioTech
ISSN: 2673-6284

Вых. Данные

Год: 2024, Том: 13, Номер: 4, Номер статьи : 57, Страниц : 14 DOI: 10.3390/biotech13040057

Ключевые слова

orthopoxviruses; monkeypox virus; phospholipase F13; p37; ST-246; NIOCH-14; chaperone; GroEL/ES; trigger factor

Авторы

Merkuleva Iuliia A. ¹ , Nikitin Vladimir N. ¹ , Belaya Tatyana D. ¹ , Mustaev Egor.A. ^2,3 , Shcherbakov Dmitriy N. ¹

Организации

1	State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia
2	Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia
3	Synchrotron Radiation Facility—Siberian Circular Photon Source “SKIF” Boreskov Institute of Catalysis of Siberian Branch of the Russian Academy of Sciences, 630559 Koltsovo, Russia

Информация о финансировании (1)

Министерство науки и высшего образования Российской Федерации (с 15 мая 2018)

075-15-2021-1355 (121120100401-9)

Heterologous protein expression often faces significant challenges, particularly when the target protein has posttranslational modifications, is toxic, or is prone to misfolding. These issues can result in low expression levels, aggregation, or even cell death. Such problems are exemplified by the expression of phospholipase p37, a critical target for chemotherapeutic drugs against pathogenic human orthopoxviruses, including monkeypox and smallpox viruses. The complex structure and broad enzymatic activity of phospholipase p37 render it toxic to host cells, necessitating specialized strategies for heterologous expression. In our study, we addressed these challenges using the vaccinia virus F13 protein as a model. We demonstrated that p37 can be effectively synthesized in E. coli as a GST fusion protein by co-expressing it with the GroEL/ES chaperone system and Trigger Factor chaperone.

Merkuleva I.A. , Nikitin V.N. , Belaya T.D. , Mustaev E.A. , Shcherbakov D.N.
The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in E. coli
BioTech. 2024. V.13. N4. 57 :1-14. DOI: 10.3390/biotech13040057 WOS Scopus CAPlus PMID OpenAlex СКИФ ID

Поступила в редакцию:	29 нояб. 2024 г.
Принята к публикации:	20 дек. 2024 г.
Опубликована в печати:	23 дек. 2024 г.
Опубликована online:	23 дек. 2024 г.

Web of science:	WOS:001386743900001
Scopus:	2-s2.0-85213024144
Chemical Abstracts:	2025:128240
PMID (PubMed):	39727494
OpenAlex:	W4405721018
СКИФ ID:	3653

БД	Цитирований
Scopus	1