Mechanism of DNA and RNA Degradation over a Photoactive TiO2@SiO2-Coated Fabric | Sciact - CRIS-система Института катализа

Mechanism of DNA and RNA Degradation over a Photoactive TiO2@SiO2-Coated Fabric Научная публикация

Журнал

International Journal of Biological Macromolecules
ISSN: 0141-8130

Вых. Данные

Год: 2025, Том: 318, Номер статьи : 145089, Страниц : 13 DOI: 10.1016/j.ijbiomac.2025.145089

Ключевые слова

Nucleic acids; Cross-contamination; Photocatalytic degradation; Self-cleaning material; Reactive oxygen species (ROS); Polymerase chain reaction (PCR)

Авторы

Solovyeva Maria ¹ , Stepanov Grigory ² , Zhuravlev Evgenii ² , Kozlov Denis ¹ , Zharkov Dmitry ² , Dvornikova Antonina ² , Selishchev Dmitry ¹

Организации

1	Boreskov Institute of Catalysis, Lavrentieva Ave. 5, 630090 Novosibirsk, Russian Federation
2	Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave. 8, 630090 Novosibirsk, Russian Federation

Информация о финансировании (1)

Министерство науки и высшего образования Российской Федерации (с 15 мая 2018)

FWUR-2024-0036

Contamination of biochemical diagnostic laboratories with impurity nucleic acids (NAs) strongly affects tests using highly sensitive microbiological methods such as polymerase chain reaction because these NAs can contaminate test samples or reagents and lead to false positive results of analysis. NAs, as biomacromolecules, remain stable on the surface of many materials for a long time, and special treatments are needed to achieve the complete degradation of NAs on the material surface. In this study, we investigated the degradation of DNA fragments, genomic DNA, ribosomal RNA, and small nuclear RNA on the UVA-irradiated surface of photoactive self-cleaning textile material prepared via the functionalization of cotton/polyester blended fabric with TiO2 nanoparticles using a silicon binder. Special techniques using enzymes that can repair the correct structure of NAs were employed to discover the mechanism of photocatalytic NA degradation over TiO2@SiO2-coated fabric. The results of this study show that the decrease in the number of NA molecules detected using PCR occurs due to the accumulation of oxidative damage to nitrogenous bases and the appearance of both single-strand and double-strand breaks in the NA backbone.

Solovyeva M. , Stepanov G. , Zhuravlev E. , Kozlov D. , Zharkov D. , Dvornikova A. , Selishchev D.
Mechanism of DNA and RNA Degradation over a Photoactive TiO2@SiO2-Coated Fabric
International Journal of Biological Macromolecules. 2025. V.318. 145089 :1-13. DOI: 10.1016/j.ijbiomac.2025.145089 WOS Scopus PMID OpenAlex

Поступила в редакцию:	12 февр. 2025 г.
Принята к публикации:	6 июн. 2025 г.
Опубликована online:	8 июн. 2025 г.
Опубликована в печати:	1 июл. 2025 г.

Web of science:	WOS:001513365100025
Scopus:	2-s2.0-105007605000
PMID (PubMed):	40494472
OpenAlex:	W4411125715

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