Differential Binding of ΔFN3 Proteins of Bifidobacterium longum GT15 and Bifidobacterium bifidum 791 to Cytokines Determined by Surface Plasmon Resonance and de Novo Molecular Modeling | Sciact - CRIS-system of Boreskov Institute of Catalysis

Differential Binding of ΔFN3 Proteins of Bifidobacterium longum GT15 and Bifidobacterium bifidum 791 to Cytokines Determined by Surface Plasmon Resonance and de Novo Molecular Modeling Full article

Journal

International Journal of Molecular Sciences
ISSN: 1422-0067 , E-ISSN: 1661-6596

Output data

Year: 2025, DOI: 10.20944/preprints202509.2197.v1

Tags

intestinal microbiota; bifidobacteria; fibronectin binding domains; recombinant ΔFN3 proteins; protein-protein interactions; cytokines; tumor necrosis factor α; gene expression

Authors

Alekseeva Maria G. ¹ , Borisevich Sophia S. ² , Yusupova Alfia R. ¹ , Reznikova Diana A. ^1,3 , Mavletova Dilara A. ¹ , Nesterov Andrey A. ¹ , Ilyina Margarita G. ² , Akimova Natalia I. ¹ , Shtil Alexander A. ⁴ , Danilenko Valery N. ¹

Affiliations

1	Vavilov Institute of General Genetics, Russian Academy of Sciences, 119333, Moscow, Russia
2	Synchrotron Radiation Facility—Siberian Circular Photon Source “SKlF” Boreskov Institute of Catalysis of Siberian Branch of the Russian Academy of Sciences, Koltsovo 630559, Russia
3	Moscow Center for Advanced Studies, 123592, Moscow, Russia
4	Blokhin National Medical Research Center of Oncology, 115522, Moscow, Russia

Bifidobacteria, a genus of obligate anaerobes, comprise a major component of the intestinal microbiota. Importantly, bifidobacteria participate in the immune reactions. These bacteria carry a species-specific operon in which the fn3 gene encodes a multifunctional protein FN3 that mediates the bacterial adhesion to the intestinal epithelium and is capable of binding individual cytokines. Bioinformatics and biochemical approaches were used to study the possible interaction of recombinant protein fragments ∆FN3 of B. longum and B. bifidum strains with cytokines TNF-α, IL-6, IL-8 and IL-10. De novo molecular modeling generated, for the first time, the structural models of species-derived ∆FN3 proteins and revealed new tentative regions for differential cytokine binding. Combined treatment with ∆FN3 and TNF-α induced TNF-α mRNA abundance in the human monocytic cell line. Altogether, these findings provide structural evidence for regulation of immune reactions by microbiota-derived proteins.

Alekseeva M.G. , Borisevich S.S. , Yusupova A.R. , Reznikova D.A. , Mavletova D.A. , Nesterov A.A. , Ilyina M.G. , Akimova N.I. , Shtil A.A. , Danilenko V.N.
Differential Binding of ΔFN3 Proteins of Bifidobacterium longum GT15 and Bifidobacterium bifidum 791 to Cytokines Determined by Surface Plasmon Resonance and de Novo Molecular Modeling
International Journal of Molecular Sciences. 2025. DOI: 10.20944/preprints202509.2197.v1 OpenAlex publication_identifier_short.sciact_skif_identifier_type

Published online:

Sep 26, 2025

OpenAlex:	W4414584858
publication_identifier.sciact_skif_identifier_type:	4180

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