The Impact of Multi-Walled Carbon Nanotubes with Different Amount of Metallic Impurities on Immunometabolic Parameters in Healthy Volunteers | Sciact - CRIS-system of Boreskov Institute of Catalysis

The Impact of Multi-Walled Carbon Nanotubes with Different Amount of Metallic Impurities on Immunometabolic Parameters in Healthy Volunteers Full article

Journal

Food and Chemical Toxicology
ISSN: 0278-6915 , E-ISSN: 1873-6351

Output data

Year: 2016, Volume: 87, Pages: 138-147 Pages count : 10 DOI: 10.1016/j.fct.2015.11.023

Tags

Gene expression, Lipid peroxidation, Metallic impurities, Multi-walled carbon nanotubes, Pathway analysis

Authors

Vitkina T.I. ¹ , Yankova V.I. ¹ , Gvozdenko T.A. ¹ , Kuznetsov V.L. ² , Krasnikov D.V. ² , Nazarenko A.V. ¹ , Chaika V.V. ³ , Smagin S.V. ³ , Tsatsakis A. ^3,4 , Engin A. ⁵ , Karakitsios S. ⁶ , Sarigiannis D. ^6,7 , Golokhvast K.S. ^1,3

Affiliations

1	Vladivostok Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration, Research Institute of Medical Climatology and Rehabilitation Treatment, Vladivostok, Russia
2	Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia
3	Far Eastern Federal University, Vladivostok, Russia
4	University of Crete, Heraklion, Greece
5	Gazi University, Faculty of Pharmacy, Department of Toxicology, 06330, Hipodrom, Ankara, Turkey
6	Aristotle University of Thessaloniki, Department of Chemical Engineering, Thessaloniki, Greece
7	Chair of Environmental Health Engineering, Institute for Advanced Study, Pavia, Italy

Funding (2)

1	Russian Science Foundation	15-14-20032
2	Far Eastern Federal University	13-06-0318

The impact of two types of multi-walled carbon nanotubes (MWCNTs) (12–14 nm) with different content of metallic impurities (purified and unpurified nanotubes) on peroxidation processes, the status of immune cells in healthy volunteers and gene expression combined to pathway analysis was studied in vitro. From the study it was shown that the main mechanism of action for both types of MWCNTs is induction of oxidative stress, the intensity of which is directly related to the amount of metallic impurities. Unpurified MWCNTs produced twice as high levels of oxidation than the purified CNTs inducing thus more intense mitochondrial dysfunction. All the above were also verified by gene expression analysis of 2 different human cellular cultures (lung epithelium and keratinoma cells) and the respective pathway analysis; modulation of genes activating the NFkB pathway is associated to inflammatory responses. This may cause a perturbation in the IL-6 signaling pathway in order to regulate inflammatory processes and compensate for apoptotic changes. A plausible hypothesis for the immunological effects observed in vivo, are considered as the result of the synergistic effect of systemic (mediated by cells of the routes of exposure) and local inflammation (blood cells).

Vitkina T.I. , Yankova V.I. , Gvozdenko T.A. , Kuznetsov V.L. , Krasnikov D.V. , Nazarenko A.V. , Chaika V.V. , Smagin S.V. , Tsatsakis A. , Engin A. , Karakitsios S. , Sarigiannis D. , Golokhvast K.S.
The Impact of Multi-Walled Carbon Nanotubes with Different Amount of Metallic Impurities on Immunometabolic Parameters in Healthy Volunteers
Food and Chemical Toxicology. 2016. V.87. P.138-147. DOI: 10.1016/j.fct.2015.11.023 WOS Scopus РИНЦ ANCAN PMID OpenAlex

Submitted:	Nov 7, 2015
Accepted:	Nov 30, 2015
Published online:	Dec 10, 2015
Published print:	Jan 1, 2016

Web of science:	WOS:000368966200015
Scopus:	2-s2.0-84949938522
Elibrary:	26794250
Chemical Abstracts:	2016:13452
Chemical Abstracts (print):	165:293921
PMID:	26683310
OpenAlex:	W2201342333

DB	Citing
Web of science	35
Scopus	42
Elibrary	50
OpenAlex	45