Deciphering How Pore Formation Causes Strain-Induced Membrane Lysis of Lipid Vesicles Full article
| Journal |
Journal of the American Chemical Society
ISSN: 0002-7863 , E-ISSN: 1520-5126 |
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| Output data | Year: 2016, Volume: 138, Number: 4, Pages: 1406-1413 Pages count : 8 DOI: 10.1021/jacs.5b12491 | ||||||||
| Tags | Peptides; Polypeptides; Pore size; Viruses | ||||||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | National Research Foundation of Singapore | NRF-NRFF2011-01 |
| 2 | National Medical Research Council | NMRC/CBRG/0005/2012 |
Abstract:
Pore formation by membrane-active antimicrobial peptides is a classic strategy of pathogen inactivation through disruption of membrane biochemical gradients. It remains unknown why some membrane-active peptides also inhibit enveloped viruses, which do not depend on biochemical gradients. Here, we employ a label-free biosensing approach based on simultaneous quartz crystal microbalance-dissipation and ellipsometry measurements in order to investigate how a pore-forming, virucidal peptide destabilizes lipid vesicles in a surface-based experimental configuration. A key advantage of the approach is that it enables direct kinetic measurement of the surface-bound peptide-to-lipid (P:L) ratio. Comprehensive experiments involving different bulk peptide concentrations and biologically relevant membrane compositions support a unified model that membrane lysis occurs at or above a critical P:L ratio, which is at least several-fold greater than the value corresponding to the onset of pore formation. That is consistent with peptide-induced pores causing additional membrane strain that leads to lysis of highly curved membranes. Collectively, the work presents a new model that describes how peptide-induced pores may destabilize lipid membranes through a membrane strain-related lytic process, and this knowledge has important implications for the design and application of membrane-active peptides. © 2016 American Chemical Society.
Cite:
Jackman J.A.
, Goh H.Z.
, Zhdanov V.P.
, Knoll W.
, Cho N-J.
Deciphering How Pore Formation Causes Strain-Induced Membrane Lysis of Lipid Vesicles
Journal of the American Chemical Society. 2016. V.138. N4. P.1406-1413. DOI: 10.1021/jacs.5b12491 WOS Scopus РИНЦ ANCAN PMID OpenAlex
Deciphering How Pore Formation Causes Strain-Induced Membrane Lysis of Lipid Vesicles
Journal of the American Chemical Society. 2016. V.138. N4. P.1406-1413. DOI: 10.1021/jacs.5b12491 WOS Scopus РИНЦ ANCAN PMID OpenAlex
Dates:
| Submitted: | Dec 2, 2015 |
| Published online: | Jan 21, 2016 |
| Published print: | Feb 3, 2016 |
Identifiers:
| Web of science: | WOS:000369558000046 |
| Scopus: | 2-s2.0-84957538645 |
| Elibrary: | 26952160 |
| Chemical Abstracts: | 2016:67340 |
| Chemical Abstracts (print): | 164:196340 |
| PMID: | 26751083 |
| OpenAlex: | W2254198722 |