Carbon-13 Radiofrequency Amplification by Stimulated Emission of Radiation of the Hyperpolarized Ketone and Hemiketal Forms of Allyl [1-13C]Pyruvate Full article
Journal |
ACS Sensors
ISSN: 2379-3694 |
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Output data | Year: 2024, Volume: 9, Number: 2, Pages: 770–780 Pages count : 11 DOI: 10.1021/acssensors.3c02075 | ||||||||||||||||||||||||||
Tags | NMR , hyperpolarization, pyruvate , C-13, parahydrogen, laser, RASER | ||||||||||||||||||||||||||
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Affiliations |
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Funding (15)
1 | National Science Foundation | CHE-1904780 |
2 | National Institute of Biomedical Imaging and Bioengineering | 1R01EB029829 |
3 | Russian Science Foundation | 21-73-10105 (121121600301-4) |
4 | Russian Science Foundation | 22-43-04426 |
5 | German Research Foundation | SFB 1479 |
6 | Wayne State University | |
7 | National Institutes of Health | R21EB025313 |
8 | National Institutes of Health | R21EB033872 |
9 | German Cancer Research Centre (DKFZ) | |
10 | Swagelok | |
11 | Universitätsklinikum Freiburg | |
12 | German Research Foundation | SCHM 3694/1-1 |
13 | German Research Foundation | SCHM 3694/2-1 |
14 | Federal Ministry of Education and Research | 13N16448 |
15 | United States Department of Energy | DE-SC0023334 |
Abstract:
13C hyperpolarized pyruvate is an emerging MRI contrast agent for sensing molecular events in cancer and other diseases with aberrant metabolic pathways. This metabolic contrast agent can be produced via several hyperpolarization techniques. Despite remarkable success in research settings, widespread clinical adoption faces substantial roadblocks because the current sensing technology utilized to sense this contrast agent requires the excitation of 13C nuclear spins that also need to be synchronized with MRI field gradient pulses. Here, we demonstrate sensing of hyperpolarized allyl [1-13C]pyruvate via the stimulated emission of radiation that mitigates the requirements currently blocking broader adoption. Specifically, 13C Radiofrequency Amplification by Stimulated Emission of Radiation (13C RASER) was obtained after pairwise addition of parahydrogen to a pyruvate precursor, detected in a commercial inductive detector with a quality factor (Q) of 32 for sample concentrations as low as 0.125 M with 13C polarization of 4%. Moreover, parahydrogen-induced polarization allowed for the preparation of a mixture of ketone and hemiketal forms of hyperpolarized allyl [1-13C]pyruvate, which are separated by 10 ppm in 13C NMR spectra. This is a good model system to study the simultaneous 13C RASER signals of multiple 13C species. This system models the metabolic production of hyperpolarized [1-13C]lactate from hyperpolarized [1-13C]pyruvate, which has a similar chemical shift difference. Our results show that 13C RASER signals can be obtained from both species simultaneously when the emission threshold is exceeded for both species. On the other hand, when the emission threshold is exceeded only for one of the hyperpolarized species, 13C stimulated emission is confined to this species only, therefore enabling the background-free detection of individual hyperpolarized 13C signals. The reported results pave the way to novel sensing approaches of 13C hyperpolarized pyruvate, potentially unlocking hyperpolarized 13C MRI on virtually any MRI system─an attractive vision for the future molecular imaging and diagnostics.
Cite:
Nantogma S.
, de Maissin H.
, Adelabu I.
, Abdurraheem A.
, Nelson C.
, Chukanov N.V.
, Salnikov O.G.
, Koptyug I.V.
, Lehmkuhl S.
, Schmidt A.B.
, Appelt S.
, Theis T.
, Chekmenev E.Y.
Carbon-13 Radiofrequency Amplification by Stimulated Emission of Radiation of the Hyperpolarized Ketone and Hemiketal Forms of Allyl [1-13C]Pyruvate
ACS Sensors. 2024. V.9. N2. P.770–780. DOI: 10.1021/acssensors.3c02075 WOS Scopus
Carbon-13 Radiofrequency Amplification by Stimulated Emission of Radiation of the Hyperpolarized Ketone and Hemiketal Forms of Allyl [1-13C]Pyruvate
ACS Sensors. 2024. V.9. N2. P.770–780. DOI: 10.1021/acssensors.3c02075 WOS Scopus
Dates:
Submitted: | Oct 1, 2023 |
Accepted: | Dec 26, 2023 |
Published online: | Jan 10, 2024 |
Published print: | Feb 23, 2024 |
Identifiers:
Web of science | WOS:001162227200001 |
Scopus | 2-s2.0-85182566219 |
OpenAlex | W4390692604 |
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