Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids Full article
Journal |
Physical Review Letters
ISSN: 0031-9007 , E-ISSN: 1079-7114 |
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Output data | Year: 2011, Volume: 107, Number: 18, Pages: 188103 Pages count : 5 DOI: 10.1103/PhysRevLett.107.188103 | ||||||||
Tags | Cell membranes; Cytology; Dissociation; Fluorescence; Fluorescence microscopy; Kinetics; Lipid bilayers; Viruses | ||||||||
Authors |
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Affiliations |
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Funding (4)
1 | Swedish Research Council | 20104041 |
2 | Stiftelsen för strategisk forskning | |
3 | VINNOVA | |
4 | Swiss National Science Foundation |
Abstract:
Glycosphingolipids are involved in the first steps of virus-cell interaction, where they mediate specific recognition of the host cell membrane. We have employed total-internal-reflection fluorescence microscopy to explore the interaction kinetics between individual unlabeled noroviruslike particles, which are attached to a glycosphingolipid-containing lipid bilayer, and fluorescent vesicles containing different types and concentrations of glycosphingolipids. Under association equilibrium, the vesicle-binding rate is found to be kinetically limited, yielding information on the corresponding activation energy. The dissociation kinetics are logarithmic over a wide range of time. The latter is explained by the vesicle-size-related distribution of the dissociation activation energy. The biological, pharmaceutical, and diagnostic relevance of the study is briefly discussed.
Cite:
Bally M.
, Gunnarsson A.
, Svensson L.
, Larson G.
, Zhdanov V.P.
, Höök F.
Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids
Physical Review Letters. 2011. V.107. N18. P.188103. DOI: 10.1103/PhysRevLett.107.188103 WOS Scopus РИНЦ
Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids
Physical Review Letters. 2011. V.107. N18. P.188103. DOI: 10.1103/PhysRevLett.107.188103 WOS Scopus РИНЦ
Files:
Full text from publisher
Dates:
Submitted: | Apr 11, 2011 |
Published print: | Oct 1, 2011 |
Published online: | Oct 28, 2011 |
Identifiers:
Web of science | WOS:000297004400007 |
Scopus | 2-s2.0-80155164903 |
Elibrary | 18011664 |
Chemical Abstracts | 2011:1422422 |
Chemical Abstracts (print) | 157:655768 |
OpenAlex | W1987796395 |