Influence of Divalent Cations on Deformation and Rupture of Adsorbed Lipid Vesicles | Sciact - CRIS-system of Boreskov Institute of Catalysis

Influence of Divalent Cations on Deformation and Rupture of Adsorbed Lipid Vesicles Full article

Journal

Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827

Output data

Year: 2016, Volume: 32, Number: 25, Pages: 6486-6495 Pages count : 10 DOI: 10.1021/acs.langmuir.6b00439

Tags

Adsorption; Deformation; Lipid bilayers; Positive ions; Quartz crystal microbalances; Silicon oxides; Surface plasmon resonance; Titanium; Titanium oxides

Authors

Dacic Marija ^1,2 , Jackman Joshua A. ^1,2 , Yorulmaz Saziye ^1,2 , Zhdanov Vladimir P. ^1,2,4 , Kasemo Bengt ⁵ , Cho Nam-Joon ^1,2,3

Affiliations

1	School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore
2	Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore
3	School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive 637459, Singapore
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia
5	Department of Physics, Chalmers University of Technology, 41296 Göteborg, Sweden

Funding (3)

1	National Research Foundation of Singapore	NRF-NRFF2011-01
2	Nanyang Technological University
3	Pomona College

The fate of adsorbed lipid vesicles on solid supports depends on numerous experimental parameters and typically results in the formation of a supported lipid bilayer (SLB) or an adsorbed vesicle layer. One of the poorly understood questions relates to how divalent cations appear to promote SLB formation in some cases. The complexity arises from the multiple ways in which divalent cations affect vesicle−substrate and vesicle−vesicle interactions as well as vesicle properties. These interactions are reflected, e.g., in the degree of deformation of adsorbed vesicles (if they do not rupture). It is, however, experimentally challenging to measure the extent of vesicle deformation in real-time. Herein, we investigated the effect of divalent cations (Mg2+, Ca2+, Sr2+) on the adsorption of zwitterionic 1,2-dioleoylsn-glycero-3-phosphocholine (DOPC) lipid vesicles onto silicon oxide- and titanium oxidecoated substrates. The vesicle adsorption process was tracked using the quartz crystal microbalance-dissipation (QCM-D) and localized surface plasmon resonance (LSPR) measurement techniques. On silicon oxide, vesicle adsorption led to SLB formation in all cases, while vesicles adsorbed but did not rupture on titanium oxide. It was identified that divalent cations promote increased deformation of adsorbed vesicles on both substrates and enhanced rupture on silicon oxide in the order Ca2+ > Mg2+ > Sr2+. The influence of divalent cations on different factors in these systems is discussed, clarifying experimental observations on both substrates. Taken together, the findings in this work offer insight into how divalent cations modulate the interfacial science of supported membrane systems.

Dacic M. , Jackman J.A. , Yorulmaz S. , Zhdanov V.P. , Kasemo B. , Cho N-J.
Influence of Divalent Cations on Deformation and Rupture of Adsorbed Lipid Vesicles
Langmuir. 2016. V.32. N25. P.6486-6495. DOI: 10.1021/acs.langmuir.6b00439 WOS Scopus РИНЦ ANCAN PMID OpenAlex

Submitted:	Feb 7, 2016
Accepted:	Apr 30, 2016
Published online:	Jun 15, 2016
Published print:	Jun 28, 2016

Web of science:	WOS:000378973300029
Scopus:	2-s2.0-84976596513
Elibrary:	26727389
Chemical Abstracts:	2016:796227
Chemical Abstracts (print):	165:62767
PMID:	27182843
OpenAlex:	W2403761724

DB	Citing
Web of science	62
Scopus	65
Elibrary	62
OpenAlex	68