Synthesis of Nanoscale TiO2 and Study of the Effect of Their Crystal Structure on Single Cell Response | Sciact - CRIS-system of Boreskov Institute of Catalysis

Synthesis of Nanoscale TiO2 and Study of the Effect of Their Crystal Structure on Single Cell Response Full article

Journal

The Scientific World Journal [electronic resource]
ISSN: 2356-6140 , E-ISSN: 1537-744X

Output data

Year: 2012, Volume: 2012, Article number : 498345, Pages count : 14 DOI: 10.1100/2012/498345

Tags

TITANIUM-DIOXIDE NANOPARTICLES; IN-VITRO; PULMONARY RESPONSE; PARTICLES; TOXICITY; NANOMATERIALS; RATS; STABILIZATION; CYTOTOXICITY; DISPERSION

Authors

Ismagilov Z.R. ¹ , Shikina N.V. ¹ , Mazurkova N.A. ² , Tsikoza L.T. ¹ , Tuzikov F.V. ¹ , Ushakov V.A. ¹ , Ishchenko A.V. ¹ , Rudina N.A. ¹ , Korneev D.V. ² , Ryabchikova E.I. ³

Affiliations

1	Boreskov Institute of Catalysis, Siberian Branch of RAS, 5, Pr. Akad. Lavrentieva, Novosibirsk 630090, Russia
2	The Vector State Research Center of Virology and Biotechnology, Rospotrebnadzor, Korp. 12, Novosibirsk oblast, Koltsovo 630559, Russia
3	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of RAS, 8, Pr. Akad. Lavrentieva, Novosibirsk 630090, Russia

Funding (4)

1	Russian Foundation for Basic Research	11-04-01408 (01201158854)
2	The Ministry of Education and Science of the Russian Federation	16.512.11.2267 (01201176480)
3	The Ministry of Education and Science of the Russian Federation	2.1.1/5642
4	Siberian Branch of the Russian Academy of Sciences	61

To study the effect of nanoscale titanium dioxide (TiO2) on cell responses, we synthesized four modifications of the TiO2 (amorphous, anatase, brookite, and rutile) capable of keeping their physicochemical characteristics in a cell culture medium. The modifications of nanoscale TiO2 were obtained by hydrolysis of TiCl4 and Ti(i-OC3H7)4 (TIP) upon variation of the synthesis conditions; their textural, morphological, structural, and dispersion characteristics were examined by a set of physicochemical methods: XRD, BET, SAXS, DLS, AFM, SEM, and HR-TEM. The effect of synthesis conditions (nature of precursor, pH, temperature, and addition of a complexing agent) on the structural-dispersion properties of TiO2 nanoparticles was studied. The hydrolysis methods providing the preparation of amorphous, anatase, brookite, and rutile modifications of TiO2 nanoparticles 3–5 nm in size were selected. Examination of different forms of TiO2 nanoparticles interaction with MDCK cells by transmission electron microscopy of ultrathin sections revealed different cell responses after treatment with different crystalline modifications and amorphous formof TiO2. The obtained results allowed us to conclude that direct contact of the nanoparticles with cell plasma membrane is the primary and critical step of their interaction and defines a subsequent response of the cell.

Ismagilov Z.R. , Shikina N.V. , Mazurkova N.A. , Tsikoza L.T. , Tuzikov F.V. , Ushakov V.A. , Ishchenko A.V. , Rudina N.A. , Korneev D.V. , Ryabchikova E.I.
Synthesis of Nanoscale TiO2 and Study of the Effect of Their Crystal Structure on Single Cell Response

The Scientific World Journal [electronic resource]. 2012. V.2012. 498345 :1-14. DOI: 10.1100/2012/498345 WOS Scopus РИНЦ ANCAN PMID OpenAlex

Full text from publisher

Submitted:	Oct 31, 2011
Accepted:	Dec 15, 2011

Web of science:	WOS:000305432500001
Scopus:	2-s2.0-84862333721
Elibrary:	17991894
Chemical Abstracts:	2012:1390144
Chemical Abstracts (print):	160:381812
PMID:	22623903
OpenAlex:	W1998730824

DB	Citing
Web of science	18
Scopus	21
Elibrary	26
OpenAlex	20