Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations | Sciact - CRIS-system of Boreskov Institute of Catalysis

Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations Full article

Journal

PLoS One
ISSN: 1932-6203

Output data

Year: 2016, Volume: 11, Number: 8, Article number : e0161915, Pages count : 13 DOI: 10.1371/journal.pone.0161915

Tags

ROTATING-WALL VESSEL; STEM-CELL; LIVER-TISSUE; PROLIFERATION; DIFFERENTIATION; GROWTH; GENERATION; MODEL; KI-67; EXPRESSION

Authors

Wang Yan ^1,2 , Kim Myung Hee ^1,2 , Tabaei Seyed R. ^1,2 , Park Jae Hyeok ^1,2 , Na Kyuhwan ³ , Chung Seok ³ , Zhdanov Vladimir P. ⁴ , Cho Nam-Joon ^1,2,5

Affiliations

1	School of Materials Science and Engineering, Nanyang Technological University, Singapore
2	Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore
3	School of Mechanical Engineering, Korea University, Seoul, Korea
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk, Russia
5	School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore

Funding (2)

1	National Research Foundation of Singapore	NRF-NRFF2011-01
2	National Research Foundation of Singapore	NRF-CRP10-2012-07

The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated lowadhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.

Wang Y. , Kim M.H. , Tabaei S.R. , Park J.H. , Na K. , Chung S. , Zhdanov V.P. , Cho N-J.
Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations

PLoS One. 2016. V.11. N8. e0161915 :1-13. DOI: 10.1371/journal.pone.0161915 WOS Scopus РИНЦ AN PMID OpenAlex

Full text from publisher

Submitted:	Apr 12, 2016
Published print:	Aug 1, 2016
Accepted:	Aug 15, 2016
Published online:	Aug 29, 2016

Web of science:	WOS:000382876700044
Scopus:	2-s2.0-84991501106
Elibrary:	27577945
Chemical Abstracts:	2017:401141
PMID:	27571565
OpenAlex:	W2517205766

DB	Citing
Web of science	22
Scopus	27
Elibrary	23
OpenAlex	29