Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity | Sciact - CRIS-система Института катализа

Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity Научная публикация

Журнал

Nature Communications
ISSN: 2041-1723

Вых. Данные

Год: 2016, Том: 7, Номер статьи : 12956, Страниц : 8 DOI: 10.1038/ncomms12956

Ключевые слова

EXTRACELLULAR VESICLES; TETHERED VESICLE; VIRUS ENTRY; TRACKING; MICROPARTICLES; EXTRUSION; CYTOMETRY; EXOSOMES; MOTION; WATER

Авторы

Block Stephan ¹ , Fast Bjorn Johansson ¹ , Lundgren Anders ^1,2 , Zhdanov Vladimir P. ^1,3 , Höök Fredrik ¹

Организации

1	Department of Physics, Division of Biological Physics, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
2	Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna 1190, Austria
3	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Информация о финансировании (3)

1	Swedish Research Council	2014-5557
2	Stiftelsen för strategisk forskning	RMA11-0104
3	Фонд Кнута и Элис Валленберг	2012.0055

Biological nanoparticles (BNPs) are of high interest due to their key role in various biological processes and use as biomarkers. BNP size and composition are decisive for their functions, but simultaneous determination of both properties with high accuracy remains challenging. Optical microscopy allows precise determination of fluorescence/scattering intensity, but not the size of individual BNPs. The latter is better determined by tracking their random motion in bulk, but the limited illumination volume for tracking this motion impedes reliable intensity determination. Here, we show that by attaching BNPs to a supported lipid bilayer, subjecting them to hydrodynamic flows and tracking their motion via surface-sensitive optical imaging enable determination of their diffusion coefficients and flow-induced drifts, from which accurate quantification of both BNP size and emission intensity can be made. For vesicles, the accuracy of this approach is demonstrated by resolving the expected radius-squared dependence of their fluorescence intensity for radii down to 15 nm

Block S. , Fast B.J. , Lundgren A. , Zhdanov V.P. , Höök F.
Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity

Nature Communications. 2016. V.7. 12956 :1-8. DOI: 10.1038/ncomms12956 WOS Scopus РИНЦ CAPlus PMID OpenAlex

Полный текст от издателя

Поступила в редакцию:	29 апр. 2016 г.
Принята к публикации:	18 авг. 2016 г.
Опубликована online:	23 сент. 2016 г.
Опубликована в печати:	1 дек. 2016 г.

Web of science:	WOS:000385445300002
Scopus:	2-s2.0-84988566343
РИНЦ:	27579115
Chemical Abstracts:	2016:1565755
PMID (PubMed):	27658367
OpenAlex:	W2340944929

БД	Цитирований
Web of science	32
Scopus	33
РИНЦ	32
OpenAlex	38