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Catalytical Properties of Arthrobacter nicotianae Cells, a Producer of Glucose Isomerase, Immobilized Inside Xerogel of Silicium Dioxide Научная публикация

Журнал Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024
Вых. Данные Год: 2009, Том: 45, Номер: 4, Страницы: 389-394 Страниц : 5 DOI: 10.1134/S0003683809040073
Ключевые слова Immobilization; Fructose; Apply Biochemistry; Monosaccharide; Immobilize Cell
Авторы Perminova L.V. 1 , Kovalenko G.A. 1,2 , Rudina N.A. 1 , Sapunova L.I. 3 , Tamkovic I.O. 3 , Lobanok A.G. 3
Организации
1 Institute of Catalysis, Siberian Division of the Russian Academy of Sciences, Novosibirsk 630090, Russia
2 Novosibirsk State University, Novosibirsk 630090, Russia
3 Institute of Microbiology National Academy of Sciences of Belarus, Minsk 220141, Belarus

Информация о финансировании (2)

1 Сибирское отделение Российской академии наук 4.4
2 Белорусский республиканский фонд фундаментальных исследований Б07CO-011

Реферат: Arthrobacter nicotianae cells, producers of glucose isomerase, were immobilized inside xerogel of silicium dioxide, and properties of the resulted heterogeneous biocatalysts were investigated in the process of isomerization of monosaccharide (glucose and fructose). The glucose isomerase activity of the resulted biocatalysts was shown to be 10 U/g, on average, taking into account the loss of the activity upon the immobilization, which amounted to 50% of the cell activity in suspension. The rate of the fructose isomerization increased linearly in the range of 55–80°C with the temperature coefficient 1.3. The biocatalysts were stable in this range; they were rapidly inactivated, however, at increasing temperature. The half-pife time of inactivation was six to seven h and five min or less at 80 and 85°C, respectively. The half-pife time of inactivation of heterogeneous biocatalysts was 50–90 h in the periodic process of isomerization of 2 M monosaccharides at 60°C in the presence of the immobilized Arthrobacter nicotianae cells.
Библиографическая ссылка: Perminova L.V. , Kovalenko G.A. , Rudina N.A. , Sapunova L.I. , Tamkovic I.O. , Lobanok A.G.
Catalytical Properties of Arthrobacter nicotianae Cells, a Producer of Glucose Isomerase, Immobilized Inside Xerogel of Silicium Dioxide
Applied Biochemistry and Microbiology. 2009. V.45. N4. P.389-394. DOI: 10.1134/S0003683809040073 WOS Scopus РИНЦ CAPlusCA OpenAlex
Оригинальная: Перминова Л.В. , Коваленко Г.А. , Рудина Н.А. , Сапунова Л.И. , Тамкович И.О. , Лобанок А.Г.
Каталитические свойства клеток продуцента глюкозоизомеразы Arthrobacter nicotianae, иммобилизованных в ксерогеле диоксида кремния
Прикладная биохимия и микробиология. 2009. Т.45. №4. С.432-438. Scopus РИНЦ
Даты:
Поступила в редакцию: 5 февр. 2008 г.
Опубликована в печати: 1 июл. 2009 г.
Опубликована online: 10 июл. 2009 г.
Идентификаторы БД:
Web of science: WOS:000267884300007
Scopus: 2-s2.0-70349294199
РИНЦ: 15311037
Chemical Abstracts: 2009:829606
Chemical Abstracts (print): 151:311600
OpenAlex: W2029252040
Цитирование в БД:
БД Цитирований
Web of science 6
Scopus 6
РИНЦ 6
OpenAlex 8
Альметрики: