Influence of Divalent Cations on Deformation and Rupture of Adsorbed Lipid Vesicles | Sciact - CRIS-system of Boreskov Institute of Catalysis

Influence of Divalent Cations on Deformation and Rupture of Adsorbed Lipid Vesicles Conference Abstracts

Conference

252nd ACS National Meeting & Exposition
21-25 Aug 2016 , Philadelphia, PA

Source

252nd American Chemical Society National Meeting & Exposition, August 21 – 25, 2016, Philadelphia, PA
Compilation, 2016.

Journal

Abstracts of Papers of the American Chemical Society
ISSN: 0065-7727

Output data

Year: 2016, Volume: 252, Article number : COLL-397, Pages count : 1

Authors

Dacic Marija ^1,3 , Jackman Joshua A. ^1,3 , Yorulmaz Saziye ^1,3 , Zhdanov Vladimir Petrovich ^3,4 , Kasemo Bengt Herbert ² , Cho Nam-Joon ^1,3

Affiliations

1	Nanyang Technol Univ, Ctr Biomimet Sensor Sci, Singapore, Singapore
2	Chalmers Univ, Dept Appl Phys, Gothenburg, Sweden
3	Nanyang Technol Univ, Sch Mat Sci & Engn, Singapore, Singapore
4	Russian Acad Sci, Boreskov Inst Catalysis, Novosibirsk, Russia

The fate of adsorbed lipid vesicles on solid supports depends on numerous exptl. parameters and typically results in the formation of a supported lipid bilayer (SLB) or an adsorbed vesicle layer. One of the poorly understood questions relates to how divalent cations appear to promote SLB formation in some cases. The complexity arises from the multiple ways in which divalent cations affect vesicle-substrate and vesicle-vesicle interactions as well as vesicle properties. These interactions are reflected, e.g., in the degree of deformation of adsorbed vesicles (if they do not rupture). It is however exptl. challenging to measure the extent of vesicle deformation in real-time. Herein, we investigated the effect of divalent cations (Mg2+ , Ca2+, Sr2+) on the adsorption of zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid vesicles onto silicon oxide- and titanium oxide-coated substrates. The vesicle adsorption process was tracked using the quartz crystal microbalance-dissipation (QCM-D) and localized surface plasmon resonance (LSPR) measurement techniques. On silicon oxide, vesicle adsorption led to SLB formation in all cases, while vesicles adsorbed but did not rupture on titanium oxide. It was identified that divalent cations promote increased deformation of adsorbed vesicles on both substrates and enhanced rupture on silicon oxide in the order Ca2+ > Mg2+ > Sr2+ . The multiple influences of divalent cations on different factors in the system are discussed, clarifying exptl. observations on both substrates. Taken together, the findings in this work offer insight into how divalent cations modulate the interfacial science of supported membrane systems.

Dacic M. , Jackman J.A. , Yorulmaz S. , Zhdanov V.P. , Kasemo B.H. , Cho N.
Influence of Divalent Cations on Deformation and Rupture of Adsorbed Lipid Vesicles
In compilation 252nd American Chemical Society National Meeting & Exposition, August 21 – 25, 2016, Philadelphia, PA. 2016. WOS AN

Published print:

Aug 1, 2016

Web of science:	WOS:000431460203055
Chemical Abstracts:	2016:1348272

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