A Technology for Pilot Production of Bacterial Cellulose from Oat Hulls Full article
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Chemical Engineering Journal
ISSN: 1385-8947 , E-ISSN: 1873-3212 |
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| Output data | Year: 2020, Volume: 383, Article number : 123128, Pages count : 10 DOI: 10.1016/j.cej.2019.123128 | ||||
| Tags | Complete-cycle pilot plant, Oat hulls, Nitric-acid pretreatment, Enzymatic hydrolysis, Medusomyces gisevii, Bacterial cellulose | ||||
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| Affiliations |
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Funding (1)
| 1 | Russian Science Foundation | 17-19-01054 |
Abstract:
The complete production cycle of bacterial cellulose (BC) was studied on a pilot scale. Oat hulls, a globally abundant agricultural residue, was utilized as the feedstock. A technology for producing BC from oat hulls has been developed herein and involves four process operations: exclusive chemical pretreatment with HNO3, enzymatic saccharification, mixed-culture fermentation, and purification. The HNO3 pretreatment was studied under atmospheric pressure at concentrations of 2–6 wt%. The optimum HNO3 concentration for pretreatment of oat hulls was found to be 4 wt%. The pretreatment was run in a 250-L vessel to furnish a pulp in 37.5% yield with 71.5% Kürschner cellulose content. The resultant pulp was well hydrolyzable: enzymatic saccharification with commercial enzymes at an initial solid loading of 30.0 g/L resulted in 79.5% reducing sugar (RS) yield. However, the test synthesis of BC using Medusomyces gisevii showed a very low yield of only 3.5%. It was therefore decided to improve the pulp with the aid of 4 wt% NaOH, whereupon oat-hull cellulose was obtained in 24% yield comprising 93.2% α-cellulose. The enzymatic saccharification of oat-hull cellulose ended with 73.5% RS yield. The test synthesis of BC exhibited 11% yield. Then, the biotechnological stages were scaled up: enzymatic saccharification of oat-hull cellulose was performed in a 100-L fermentor, and BC synthesis in the resultant hydrolyzate was run in a Binder KB-400 incubator. After purification, the BC yield was 10% of the RS concentration. BC was composed of 100% cellulose Iα-allomorph and had 93% crystallinity index. The BC production from oat hulls was thus scaled up successfully with an optimistic BC yield: 80.5 tons of 98%-wet hydrogel per 100 tons of oat hulls.
Cite:
Skiba E.A.
, Budaeva V.V.
, Ovchinnikova E.V.
, Gladysheva E.K.
, Kashcheyeva E.I.
, Pavlov I.N.
, Sakovich G.V.
A Technology for Pilot Production of Bacterial Cellulose from Oat Hulls
Chemical Engineering Journal. 2020. V.383. 123128 :1-10. DOI: 10.1016/j.cej.2019.123128 WOS Scopus РИНЦ AN OpenAlex
A Technology for Pilot Production of Bacterial Cellulose from Oat Hulls
Chemical Engineering Journal. 2020. V.383. 123128 :1-10. DOI: 10.1016/j.cej.2019.123128 WOS Scopus РИНЦ AN OpenAlex
Dates:
| Submitted: | Sep 11, 2019 |
| Accepted: | Oct 10, 2019 |
| Published online: | Oct 12, 2019 |
| Published print: | Mar 1, 2020 |
Identifiers:
| Web of science: | WOS:000504404800018 |
| Scopus: | 2-s2.0-85073813471 |
| Elibrary: | 41714504 |
| Chemical Abstracts: | 2019:1996501 |
| OpenAlex: | W2979292905 |