Abstract: It is shown that an increase in the amplitude of QCM shear oscillations during frequency scanning around the resonance frequency is accompanied (at a definite voltage) by distortions in the amplitude–frequency dependence for QCM. We demonstrated that these distortions are connected to the rupture of macromolecules from the QCM surface. It is shown that the identification of the rupture of particles and macromolecules from the QCM surface can be carried out by relying on the analysis of these distortions of the amplitude–frequency dependence. The distortions were distinguished as a signal. The number of broken bonds can be estimated from the value of this distortion signal, and the threshold voltage applied to the system can be used to estimate the rupture force to high accuracy. Using the proposed method, we estimated the strength of a physical bond, which was 3 pN. This procedure can be useful for studying biological objects and represents an advanced step in the development of the REVS (rupture event scanning) technique.

Cite: Dultsev F.N. , Kolosovsky E.A. , Mik I.A.
New Procedure to Record the Rupture of Bonds between Macromolecules and the Surface of the Quartz Crystal Microbalance (QCM)
Langmuir. 2012. V.28. N39. P.13793-13797. DOI: 10.1021/la302907r WOS Scopus РИНЦ ANCAN OpenAlex

Dates:

Submitted:	Jul 22, 2012
Accepted:	Sep 11, 2012
Published online:	Sep 14, 2012
Published print:	Oct 2, 2012

Identifiers:

Web of science:	WOS:000309431100005
Scopus:	2-s2.0-84867058977
Elibrary:	20489711
Chemical Abstracts:	2012:1341641
Chemical Abstracts (print):	157:493279
OpenAlex:	W2317265512

Citing:

DB	Citing
Web of science	9
Scopus	9
OpenAlex	11

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