Single X-ray Irradiation Modulates Proteoglycan Expression in Brain Tissue: Investigation Using Mouse Model | Sciact - CRIS-system of Boreskov Institute of Catalysis

Single X-ray Irradiation Modulates Proteoglycan Expression in Brain Tissue: Investigation Using Mouse Model Full article

Journal

Molecular Biology Reports
ISSN: 0301-4851

Output data

Year: 2020, Volume: 47, Number: 7, Pages: 5657 - 5663 Pages count : 7 DOI: 10.1007/s11033-020-05578-1

Tags

Radiotherapy animal model · Mouse brain irradiation · Tissue morphology · Extracellular matrix · Proteoglycan expression

Authors

Politko Maxim O. ¹ , Prokaeva Anna I. ¹ , Pashkovskaya Oxana A. ² , Kuper Konstantin E. ³ , Zheravin Alexander A. ² , Kliver Evgenii E. ² , Tsidulko Alexandra Y. ¹ , Aidagulova Svetlana V. ⁴ , Grigorieva Elvira V. ¹

Affiliations

1	Institute of Molecular Biology and Biophysics FRC FTM, Timakova 2/12, Novosibirsk 630117, Russia
2	Meshalkin National Medical Research Center, Novosibirsk, Russia
3	Budker Institute of Nuclear Physics SB RAS, Novosibirsk, Russia
4	Novosibirsk State Medical University, Novosibirsk, Russia

Radiotherapy is an integral part of glioblastoma treatment affecting both cancer cells and tumour microenvironment, where proteoglycans (PGs) are key extracellular components. However, the molecular effects of radiotherapy on PGs expression and functional activity in brain tissue are poorly understood. Here, we aimed to study the short-term effects of X-ray irradiation on PGs expression in normal brain tissue in mouse model in vivo. Two-month-old male CBL/6Bl mice (n = 54) were used in this study, animals’ brains were irradiated using either research synchrotron VEPP-4 or clinical linear accelerator ElektaAxesse. Control (n = 18) and irradiated (n = 36) brain tissues were analysed at 24 h, 48 h and 72 h after irradiation. Morphology of the cortex and hippocampus was accessed by H&E staining, and expression of PGs (syndecan-1, glypican-1, HSPG2/perlecan, versican, brevican, neurocan, NG2/CSPG4, CD44, decorin, biglycan) was determined by RT-PCR. Single irradiation of mouse brain with a 7 Gy dose did not affect tissue morphology and mRNA levels of most highly-expressed PGs decorin and neurocan, although resulted in significant downregulation of brevican (3–10-fold) and NG2/CSPG4 (8–9-fold) expression both in cerebral cortex and subcortex. Research synchrotron and clinical linear accelerators demonstrated minor variability in their effects. Single X-ray irradiation with a 7 Gy dose does not significantly affect the mouse brain tissue morphology but selectively decreases expression levels of some PGs. The downregulation of brevican and NG2/CSPG4 but not decorin and neurocan reflects alteration of extracellular matrix in irradiated brain tissue, which might contribute to the formation of a permissive microenvironment for glioblastoma relapse development. © 2020, Springer Nature B.V.

Politko M.O. , Prokaeva A.I. , Pashkovskaya O.A. , Kuper K.E. , Zheravin A.A. , Kliver E.E. , Tsidulko A.Y. , Aidagulova S.V. , Grigorieva E.V.
Single X-ray Irradiation Modulates Proteoglycan Expression in Brain Tissue: Investigation Using Mouse Model
Molecular Biology Reports. 2020. V.47. N7. P.5657 - 5663. DOI: 10.1007/s11033-020-05578-1 WOS Scopus РИНЦ ANAN OpenAlex

Submitted:	Apr 16, 2020
Accepted:	Jun 5, 2020
Published online:	Jun 8, 2020
Published print:	Jul 1, 2020

Web of science:	WOS:000539360500003
Scopus:	2-s2.0-85086082628
Elibrary:	43294476
Chemical Abstracts:	2020:1117077 \| 173:303877
OpenAlex:	W3034066855

DB	Citing
Web of science	4
Scopus	4
OpenAlex	4