Unraveling How Cholesterol Affects Multivalency-Induced Membrane Deformation of Sub-100 nm Lipid Vesicles Full article
| Journal |
Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827 |
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| Output data | Year: 2022, Volume: 38, Number: 51, Pages: 15950-15959 Pages count : 10 DOI: 10.1021/acs.langmuir.2c02252 | ||||||||||
| Tags | water permeability; delivery; nanoparticles; model | ||||||||||
| Authors |
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| Affiliations |
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Funding (5)
| 1 | National Research Foundation of Korea | 2020R1C1C1004385 |
| 2 | National Research Foundation of Korea | 2021R1A4A1032782 |
| 3 | National Research Foundation of Korea | 2020K1A3A1A39112724 |
| 4 | Ministry of Health and Welfare | HI19C1328 |
| 5 | Sungkyunkwan University |
Abstract:
Cholesterol plays a critical role in modulating the lipid membrane properties of biological and biomimetic systems and recent attention has focused on its role in the functions of sub-100 nm lipid vesicles and lipid nanoparticles. These functions often rely on multivalent ligand–receptor interactions involving membrane attachment and dynamic shape transformations while the extent to which cholesterol can influence such interaction processes is largely unknown. To address this question, herein, we investigated the attachment of sub-100 nm lipid vesicles containing varying cholesterol fractions (0–45 mol %) to membrane-mimicking supported lipid bilayer (SLB) platforms. Biotinylated lipids and streptavidin proteins were used as model ligands and receptors, respectively, while the localized surface plasmon resonance sensing technique was employed to track vesicle attachment kinetics in combination with analytical modeling of vesicle shape changes. Across various conditions mimicking low and high multivalency, our findings revealed that cholesterol-containing vesicles could bind to receptor-functionalized membranes but underwent appreciably less multivalency-induced shape deformation than vesicles without cholesterol, which can be explained by a cholesterol-mediated increase in membrane bending rigidity. Interestingly, the extent of vesicle deformation that occurred in response to increasingly strong multivalent interactions was less pronounced for vesicles with greater cholesterol fraction. The latter trend was rationalized by taking into account the strong dependence of the membrane bending energy on the area of the vesicle–SLB contact region and such insights can aid the engineering of membrane-enveloped nanoparticles with tailored biophysical properties.
Cite:
Park H.
, Sut T.N.
, Yoon B.K.
, Zhdanov V.P.
, Cho N-J.
, Jackman J.A.
Unraveling How Cholesterol Affects Multivalency-Induced Membrane Deformation of Sub-100 nm Lipid Vesicles
Langmuir. 2022. V.38. N51. P.15950-15959. DOI: 10.1021/acs.langmuir.2c02252 WOS Scopus РИНЦ ANCAN PMID OpenAlex
Unraveling How Cholesterol Affects Multivalency-Induced Membrane Deformation of Sub-100 nm Lipid Vesicles
Langmuir. 2022. V.38. N51. P.15950-15959. DOI: 10.1021/acs.langmuir.2c02252 WOS Scopus РИНЦ ANCAN PMID OpenAlex
Dates:
| Submitted: | Aug 19, 2022 |
| Accepted: | Dec 14, 2022 |
| Published online: | Dec 14, 2022 |
| Published print: | Dec 27, 2022 |
Identifiers:
| Web of science: | WOS:000898894200001 |
| Scopus: | 2-s2.0-85144302383 |
| Elibrary: | 50420622 |
| Chemical Abstracts: | 2022:3092634 |
| Chemical Abstracts (print): | 181:139132 |
| PMID: | 36515977 |
| OpenAlex: | W4311473774 |