Hydroxyapatite Double Substituted with Zinc and Silicate Ions: Possibility of Mechanochemical Synthesis and In Vitro Properties | Sciact - CRIS-system of Boreskov Institute of Catalysis

Hydroxyapatite Double Substituted with Zinc and Silicate Ions: Possibility of Mechanochemical Synthesis and In Vitro Properties Full article

Journal

Materials
ISSN: 1996-1944

Output data

Year: 2023, Volume: 16, Number: 4, Article number : 1385, Pages count : 14 DOI: 10.3390/ma16041385

Tags

hydroxyapatite; co-substitution; doping; zinc; silicone; cytotoxicity; biocompatibility

Authors

Makarova Svetlana V. ¹ , Bulina Natalia V. ¹ , Golubeva Yuliya A. ² , Klyushova Lyubov S. ^3,4 , Dumchenko Natalya B. ⁵ , Shatskaya Svetlana S. ¹ , Ishchenko Arcady V. ⁶ , Khvostov Mikhail V. ^1,7 , Dudina Dina V. ^1,8

Affiliations

1	Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia
2	Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia
3	Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia
4	Institute of Molecular Biology and Biophysics of Federal State Budget Scientific Institution “Federal Research Center of Fundamental and Translational Medicine” (IMBB FRC FTM), 630060 Novosibirsk, Russia
5	State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance in Consumer Rights Protection and Human Well-being, 630559 Koltsovo, Russia
6	G.K. Boreskov Institute of Catalysis, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia
7	Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia
8	Lavrentyev Institute of Hydrodynamics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia

Funding (3)

1	Ministry of Science and Higher Education of the Russian Federation	075-00521-21-00 (121032500064-8) (FWUS-2021-0009)
2	Ministry of Science and Higher Education of the Russian Federation	075-15-2021-691
3	Ministry of Science and Higher Education of the Russian Federation	075-12-2021-697

In this study, the mechanochemical synthesis of substituted hydroxyapatite (HA) containing zinc and silicon ions having a chemical formula of Ca10−xZnx(PO4)6−x(SiO4)x(OH)2−x, where x = 0.2, 0.6, 1.0, 1.5, and 2.0, was carried out. The synthesized materials were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, and inductively coupled plasma spectroscopy. We found that HA co-substituted with zinc and silicate formed up to x = 1.0. At higher concentrations of the substituents, the formation of large amounts of an amorphous phase was observed. The cytotoxicity and biocompatibility of the co-substituted HA was studied in vitro on Hek293 and MG-63 cell lines. The HA co-substituted with zinc and silicate demonstrated high biocompatibility; the lowest cytotoxicity was observed at x = 0.2. For this composition, good proliferation of MG-63 osteoblast-like cells and an increased solubility compared with that of HA were detected. These properties allow us to recommend the synthesized material for medical applications, namely, for the restoration of bone tissue and manufacture of biodegradable implants.

Makarova S.V. , Bulina N.V. , Golubeva Y.A. , Klyushova L.S. , Dumchenko N.B. , Shatskaya S.S. , Ishchenko A.V. , Khvostov M.V. , Dudina D.V.
Hydroxyapatite Double Substituted with Zinc and Silicate Ions: Possibility of Mechanochemical Synthesis and In Vitro Properties
Materials. 2023. V.16. N4. 1385 :1-14. DOI: 10.3390/ma16041385 WOS Scopus РИНЦ AN PMID OpenAlex

Submitted:	Nov 28, 2022
Accepted:	Feb 2, 2023
Published print:	Feb 7, 2023
Published online:	Feb 7, 2023

Web of science:	WOS:000941598200001
Scopus:	2-s2.0-85149254132
Elibrary:	54019903
Chemical Abstracts:	2023:398666
PMID:	36837015
OpenAlex:	W4319601159

DB	Citing
Scopus	4
Elibrary	6
OpenAlex	5
Web of science	5