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Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection Научная публикация

Журнал Polymers
, E-ISSN: 2073-4360
Вых. Данные Год: 2023, Том: 15, Номер: 15, Номер статьи : 3202, Страниц : 20 DOI: 10.3390/polym15153202
Ключевые слова electrospun scaffold; DNA delivery; plasmid DNA; nanoparticle; DNA release; cell transfection; manipulating cellular behaviors
Авторы Chernonosova Vera 1 , Khlebnikova Marianna 1 , Popova Victoriya 1 , Starostina Ekaterina 2 , Kiseleva Elena 3 , Chelobanov Boris 1 , Kvon Ren 4 , Dmitrienko Elena 1 , Laktionov Pavel 1
Организации
1 Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia
2 State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia
3 Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia
4 Boreskov Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia

Информация о финансировании (1)

1 Министерство науки и высшего образования Российской Федерации (с 15 мая 2018) 075-00404-21-00 (121031300042-1)

Реферат: Biomaterial-mediated, spatially localized gene delivery is important for the development of cell-populated scaffolds used in tissue engineering. Cells adhering to or penetrating into such a scaffold are to be transfected with a preloaded gene that induces the production of secreted proteins or cell reprogramming. In the present study, we produced silica nanoparticles-associated pDNA and electrospun scaffolds loaded with such nanoparticles, and studied the release of pDNA from scaffolds and cell-to-scaffold interactions in terms of cell viability and pDNA transfection efficacy. The pDNA-coated nanoparticles were characterized with dynamic light scattering and transmission electron microscopy. Particle sizes ranging from 56 to 78 nm were indicative of their potential for cell transfection. The scaffolds were characterized using scanning electron microscopy, X-ray photoelectron spectroscopy, stress-loading tests and interaction with HEK293T cells. It was found that the properties of materials and the pDNA released vary, depending on the scaffold’s composition. The scaffolds loaded with pDNA-nanoparticles do not have a pronounced cytotoxic effect, and can be recommended for cell transfection. It was found that (pDNA-NPs) + PEI9-loaded scaffold demonstrates good potential for cell transfection. Thus, electrospun scaffolds suitable for the transfection of inhabiting cells are eligible for use in tissue engineering.
Библиографическая ссылка: Chernonosova V. , Khlebnikova M. , Popova V. , Starostina E. , Kiseleva E. , Chelobanov B. , Kvon R. , Dmitrienko E. , Laktionov P.
Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection
Polymers. 2023. V.15. N15. 3202 :1-20. DOI: 10.3390/polym15153202 WOS Scopus РИНЦ CAPlus PMID OpenAlex
Даты:
Поступила в редакцию: 16 июн. 2023 г.
Принята к публикации: 26 июл. 2023 г.
Опубликована online: 27 июл. 2023 г.
Опубликована в печати: 1 авг. 2023 г.
Идентификаторы БД:
Web of science: WOS:001046322400001
Scopus: 2-s2.0-85167796551
РИНЦ: 54361431
Chemical Abstracts: 2023:1717223
PMID (PubMed): 37571096
OpenAlex: W4385348825
Цитирование в БД:
БД Цитирований
OpenAlex 3
Web of science 2
РИНЦ 2
Scopus 3
Альметрики: