StenM_174: A Novel Podophage That Infects a Wide Range of Stenotrophomonas spp. and Suggests a New Subfamily in the Family Autographiviridae | Sciact - CRIS-system of Boreskov Institute of Catalysis

StenM_174: A Novel Podophage That Infects a Wide Range of Stenotrophomonas spp. and Suggests a New Subfamily in the Family Autographiviridae Full article

Journal

Viruses
, E-ISSN: 1999-4915

Output data

Year: 2024, Volume: 16, Number: 1, Article number : 18, Pages count : 17 DOI: 10.3390/v16010018

Tags

Autographiviridae; Stenotrophomonas maltophilia; virulent phage; comparative genomics; tail spike; receptor-binding protein; structural modeling; AlphaFold

Authors

Morozova Vera V. ¹ , Yakubovskij Vyacheslav I. ^2,1 , Baykov Ivan K. ^1,3 , Kozlova Yuliya N. ¹ , Tikunov Artem Yu. ¹ , Babkin Igor V. ¹ , Bardasheva Alevtina V. ¹ , Zhirakovskaya Elena V. ¹ , Tikunova Nina V. ¹

Affiliations

1	Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia
2	Faculty of Natural Sciences, Novosibirsk State University, Novosibirsk 630090, Russia
3	Shared Research Facility “Siberian Circular Photon Source” (SRF “SKIF”) of Boreskov Institute of Catalysis SB RAS, Novosibirsk 630090, Russia

Stenotrophomonas maltophilia was discovered as a soil bacterium associated with the rhizosphere. Later, S. maltophilia was found to be a multidrug-resistant hospital-associated pathogen. Lytic bacteriophages are prospective antimicrobials; therefore, there is a need for the isolation and characterization of new Stenotrophomonas phages. The phage StenM_174 was isolated from litter at a poultry farm using a clinical strain of S. maltophilia as the host. StenM_174 reproduced in a wide range of clinical and environmental strains of Stenotrophomonas, mainly S. maltophilia, and it had a podovirus morphotype. The length of the genomic sequence of StenM_174 was 42,956 bp, and it contained 52 putative genes. All genes were unidirectional, and 31 of them encoded proteins with predicted functions, while the remaining 21 were identified as hypothetical ones. Two tail spike proteins of StenM_174 were predicted using AlphaFold2 structural modeling. A comparative analysis of the genome shows that the Stenotrophomonas phage StenM_174, along with the phages Ponderosa, Pepon, Ptah, and TS-10, can be members of the new putative genus Ponderosavirus in the Autographiviridae family. In addition, the analyzed data suggest a new subfamily within this family.

Morozova V.V. , Yakubovskij V.I. , Baykov I.K. , Kozlova Y.N. , Tikunov A.Y. , Babkin I.V. , Bardasheva A.V. , Zhirakovskaya E.V. , Tikunova N.V.
StenM_174: A Novel Podophage That Infects a Wide Range of Stenotrophomonas spp. and Suggests a New Subfamily in the Family Autographiviridae
Viruses. 2024. V.16. N1. 18 :1-17. DOI: 10.3390/v16010018 WOS Scopus AN PMID OpenAlex

Submitted:	Nov 11, 2023
Accepted:	Dec 20, 2023
Published online:	Dec 21, 2023
Published print:	Jan 1, 2024

Web of science:	WOS:001152880600001
Scopus:	2-s2.0-85183755773
Chemical Abstracts:	2024:285144
PMID:	38275953
OpenAlex:	W4390048725

DB	Citing
Scopus	2
Web of science	2