The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in E. coli | Sciact - CRIS-system of Boreskov Institute of Catalysis

The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in E. coli Full article

Journal

BioTech
ISSN: 2673-6284

Output data

Year: 2024, Volume: 13, Number: 4, Article number : 57, Pages count : 14 DOI: 10.3390/biotech13040057

Tags

orthopoxviruses; monkeypox virus; phospholipase F13; p37; ST-246; NIOCH-14; chaperone; GroEL/ES; trigger factor

Authors

Merkuleva Iuliia A. ¹ , Nikitin Vladimir N. ¹ , Belaya Tatyana D. ¹ , Mustaev Egor.A. ^2,3 , Shcherbakov Dmitriy N. ¹

Affiliations

1	State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia
2	Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia
3	Synchrotron Radiation Facility—Siberian Circular Photon Source “SKIF” Boreskov Institute of Catalysis of Siberian Branch of the Russian Academy of Sciences, 630559 Koltsovo, Russia

Funding (1)

Ministry of Science and Higher Education of the Russian Federation

075-15-2021-1355 (121120100401-9)

Heterologous protein expression often faces significant challenges, particularly when the target protein has posttranslational modifications, is toxic, or is prone to misfolding. These issues can result in low expression levels, aggregation, or even cell death. Such problems are exemplified by the expression of phospholipase p37, a critical target for chemotherapeutic drugs against pathogenic human orthopoxviruses, including monkeypox and smallpox viruses. The complex structure and broad enzymatic activity of phospholipase p37 render it toxic to host cells, necessitating specialized strategies for heterologous expression. In our study, we addressed these challenges using the vaccinia virus F13 protein as a model. We demonstrated that p37 can be effectively synthesized in E. coli as a GST fusion protein by co-expressing it with the GroEL/ES chaperone system and Trigger Factor chaperone.

Merkuleva I.A. , Nikitin V.N. , Belaya T.D. , Mustaev E.A. , Shcherbakov D.N.
The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in E. coli
BioTech. 2024. V.13. N4. 57 :1-14. DOI: 10.3390/biotech13040057 WOS Scopus AN PMID OpenAlex publication_identifier_short.sciact_skif_identifier_type

Submitted:	Nov 29, 2024
Accepted:	Dec 20, 2024
Published print:	Dec 23, 2024
Published online:	Dec 23, 2024

Web of science:	WOS:001386743900001
Scopus:	2-s2.0-85213024144
Chemical Abstracts:	2025:128240
PMID:	39727494
OpenAlex:	W4405721018
publication_identifier.sciact_skif_identifier_type:	3653

DB	Citing
Scopus	1