Metabolomic and Gene Networks Approaches Reveal the Role of Mitochondrial Membrane Proteins in Response of Human Melanoma Cells to THz Radiation | Sciact - CRIS-system of Boreskov Institute of Catalysis

Metabolomic and Gene Networks Approaches Reveal the Role of Mitochondrial Membrane Proteins in Response of Human Melanoma Cells to THz Radiation Full article

Journal

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
ISSN: 1388-1981 , E-ISSN: 1879-2618

Output data

Year: 2025, Volume: 1870, Number: 2, Article number : 159595, Pages count : DOI: 10.1016/j.bbalip.2025.159595

Authors

Butikova Ekaterina A. ^1,2 , Basov Nikita V. ^1,3 , Rogachev Artem D. ^1,3 , Gaisler Evgeniy V. ¹ , Ivanisenko Vladimir A. ^1,4 , Demenkov Pavel S. ⁴ , Makarova Aelita-Luiza A. ^1,4 , Ivanisenko Timofey V. ⁴ , Razumov Ivan A. ^1,4,5 , Kolomeyets Daria A. ⁵ , Cheresiz Sergey V. ¹ , Solovieva Olga I. ^1,4,5 , Larionov Kirill P. ⁶ , Sotnikova Yulia S. ^1,3,6 , Patrushev Yuri V. ^1,6 , Kolchanov Nikolay A. ⁴ , Pokrovsky Andrey G. ¹ , Vinokurov Nikolay A. ⁵ , Kanygin Vladimir V. ^1,5 , Popik Vasiliy M. ⁵ , Shevchenko Oleg A. ⁵

Affiliations

1	Novosibirsk State University, Pirogova Str., 2, 630090 Novosibirsk, Russia
2	Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics SB RAS, Timakova str.,2, 630060 Novosibirsk, Russia
3	N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Acad. Lavrentiev Ave., 9, 630090 Novosibirsk, Russia
4	Institute of Cytology and Genetics SB RAS, Acad. Lavrentiev Ave.,10, 630090 Novosibirsk, Russia
5	Budker Institute of Nuclear Physics SB RAS, Acad. Lavrentiev Ave.,9, 630090 Novosibirsk, Russia
6	Boreskov Institute of Catalysis, Acad. Lavrentiev Ave., 5, 630090 Novosibirsk, Russia

Funding (6)

1	Russian Science Foundation	19-72-202
2	Ministry of Science and Higher Education of the Russian Federation	FSUS-2020-0035
3	Ministry of Science and Higher Education of the Russian Federation	075-00365-24-00
4	Ministry of Science and Higher Education of the Russian Federation	FWUR-2024-0032
5	Ministry of Science and Higher Education of the Russian Federation	FWNR-2022-0020 (122042000126-6)
6	Ministry of Science and Higher Education of the Russian Federation	БС 2020-0039-1

Terahertz (THz) radiation has gained attention due to technological advancements, but its biological effects remain unclear. We investigated the impact of 2.3 THz radiation on SK-MEL-28 cells using metabolomic and gene network analysis. Forty metabolites, primarily related to purine, pyrimidine synthesis and breakdown pathways, were significantly altered post-irradiation. Lipids, such as ceramides and phosphatidylcholines, were also affected. Gene network reconstruction and analysis identified key regulators of the enzymes involved in biosynthesis and degradation of significantly altered metabolites. Mitochondrial membrane components, such as the respiratory chain complex, the proton-transporting ATP synthase complex, and components of lipid rafts reacted to THz radiation. We propose that THz radiation induces reversible disruption of the lipid raft macromolecular structure, thereby altering mitochondrial molecule transport while maintaining protein integrity, which explains the high cell survival rate. Our findings enhance the understanding of THz biological effects and emphasize the role of membrane components in the cellular response to THz radiation.

Butikova E.A. , Basov N.V. , Rogachev A.D. , Gaisler E.V. , Ivanisenko V.A. , Demenkov P.S. , Makarova A-L.A. , Ivanisenko T.V. , Razumov I.A. , Kolomeyets D.A. , Cheresiz S.V. , Solovieva O.I. , Larionov K.P. , Sotnikova Y.S. , Patrushev Y.V. , Kolchanov N.A. , Pokrovsky A.G. , Vinokurov N.A. , Kanygin V.V. , Popik V.M. , Shevchenko O.A.
Metabolomic and Gene Networks Approaches Reveal the Role of Mitochondrial Membrane Proteins in Response of Human Melanoma Cells to THz Radiation
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2025. V.1870. N2. 159595 . DOI: 10.1016/j.bbalip.2025.159595 WOS Scopus РИНЦ OpenAlex

Submitted:	Jun 2, 2024
Accepted:	Jan 15, 2025
Published online:	Jan 20, 2025
Published print:	Mar 1, 2025

Web of science:	WOS:001414640300001
Scopus:	2-s2.0-85215815043
Elibrary:	80236945
OpenAlex:	W4406598373

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