Dynamics of Phosphatidylcholines in the Bronchoalveolar Lavage of Mice After the Introduction of Essential Phospholipids | Sciact - CRIS-система Института катализа

Dynamics of Phosphatidylcholines in the Bronchoalveolar Lavage of Mice After the Introduction of Essential Phospholipids Научная публикация

Журнал

Biomedical Chromatography
ISSN: 0269-3879 , E-ISSN: 1099-0801

Вых. Данные

Год: 2025, Том: 39, Номер: 12, Номер статьи : e70259, Страниц : DOI: 10.1002/bmc.70259

Ключевые слова

aerosol administration; bronchoalveolar lavage; Essentiale; LC–MS/MS; lung surfactant; phosphatidylcholines; pulmonary drug delivery

Авторы

Yurin V.V. ¹ , An'kov S.V. ² , Bogatishcheva K.S . ² , Sotnikova M.A. ^1,2 , Basov N.V . ^1,2 , Rogachev A.D. ^1,2 , Osechkova A .E . ² , Sotnikova Yu.S . ^1,2,3 , Patrushev Y.V . ^1,3 , Prokopyeva E .A. ¹ , Dolgova N.A . ¹ , Tolstikova T.G . ^1,2 , Dultseva G.G . ⁴ , Grishin O.V . ¹ , Valiulin S.V. ⁴ , Salakhutdinov N.F. ² , Pokrovsky A.G. ¹

Организации

1	Novosibirsk State University, Novosibirsk, Russia
2	Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Novosibirsk, Russia
3	Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia
4	Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, Russia

Информация о финансировании (4)

1	Министерство науки и высшего образования Российской Федерации (с 15 мая 2018)	FWUR-2024-0032
2	Министерство науки и высшего образования Российской Федерации (с 15 мая 2018)	FWGF-2021-0004
3	Министерство науки и высшего образования Российской Федерации (с 15 мая 2018)	FSUS-2020-0035
4	Министерство науки и высшего образования Российской Федерации (с 15 мая 2018)	075-00365-25-00

Lung surfactant is a lipid–protein complex essential for maintaining alveolar stability by reducing surface tension. In surfactant-deficient states such as pneumonia or acute respiratory distress syndrome, alveolar collapse and impaired gas exchange may occur. Exogenous supplementation of surfactant phospholipids has been proposed to improve lung function. In this work, we performed LC–MS/MS analysis of phosphatidylcholines from a commercially available Essentiale phospholipid preparation in bronchoalveolar lavage (BAL) fluid. The dynamics of BAL phosphatidylcholine levels in mice following intravenous and aerosol administration of Essentiale were investigated for the first time, revealing the time course and extent of pulmonary accumulation for each route. A 20-min aerosol inhalation of Essentiale (≈3 mg/kg phospholipids) achieved BAL phosphatidylcholine concentrations comparable to those obtained with a 500 mg/kg intravenous dose. Repeated daily intravenous dosing caused a gradual increase in BAL phosphatidylcholine over 3–4 days, whereas prolonged aerosol exposure beyond 20 min did not further elevate BAL levels, likely due to uptake by alveolar cells. These findings highlight aerosol inhalation as a more efficient delivery method for surfactant phospholipids and suggest an optimal dosing regimen (≈ 3 mg/kg, 20 min) for adjunctive therapy in conditions associated with surfactant deficiency.

Yurin V.V. , An'kov S.V. , Bogatishcheva K.S. , Sotnikova M.A. , Basov N.V. , Rogachev A.D. , Osechkova A.E. , Sotnikova Y.S. , Patrushev Y.V. , Prokopyeva E.A. , Dolgova N.A. , Tolstikova T.G. , Dultseva G.G. , Grishin O.V. , Valiulin S.V. , Salakhutdinov N.F. , Pokrovsky A.G.
Dynamics of Phosphatidylcholines in the Bronchoalveolar Lavage of Mice After the Introduction of Essential Phospholipids
Biomedical Chromatography. 2025. V.39. N12. e70259 . DOI: 10.1002/bmc.70259 Scopus OpenAlex

Поступила в редакцию:	10 мая 2025 г.
Принята к публикации:	16 окт. 2025 г.
Опубликована online:	11 нояб. 2025 г.
Опубликована в печати:	1 дек. 2025 г.

Scopus:	2-s2.0-105021338319
OpenAlex:	W7104980870

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