Spectrally Encoded Microspheres for Immunofluorescence Analysis
Обзор
| Общая информация |
Язык:
Английский,
Жанр:
Обзор (Review),
Статус опубликования:
Опубликована,
Оригинальность:
Оригинальная
|
| Журнал |
Journal of Applied Polymer Science
ISSN: 0021-8995
, E-ISSN: 1097-4628
|
| Вых. Данные |
Год: 2021,
Том: 138,
Номер: 8,
Номер статьи
: 49890,
Страниц
: 21
DOI:
10.1002/app.49890
|
| Ключевые слова |
bioengineering; biomedical applications; functionalization of polymers; synthesis and processing techniques |
| Авторы |
Sankova Natalya
1,2
,
Shalaev Pavel
3,4,5
,
Semeykina Viktoriya
1,2
,
Dolgushin Sergey
3,4
,
Odintsova Elena
6
,
Parkhomchuk Ekaterina
1,2
|
| Организации |
| 1 |
Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russian Federation
|
| 2 |
Boreskov Institute of Catalysis SB RAS, Group of template synthesis, Novosibirsk, Russian Federation
|
| 3 |
Gamaleya Research Center of Epidemiology and Microbiology, Translational Biomedicine Laboratory, Moscow, Russian Federation
|
| 4 |
Aivok LLC, Moscow, Russian Federation
|
| 5 |
National Research University of Electronic Technology, Institute of Biomedical Systems, Moscow, Russian Federation
|
| 6 |
Sechenov First Moscow State Medical University, Moscow, Russian Federation
|
|
Информация о финансировании (1)
|
1
|
Федеральное агентство научных организаций России
|
0303-2016-0010
|
A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis. © 2020 Wiley Periodicals LLC