Interaction of Virus-Like Particles with Vesicles Containing Glycolipids: Kinetics of Detachment Научная публикация
Журнал |
The Journal of Physical Chemistry B
ISSN: 1520-6106 , E-ISSN: 1520-5207 |
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Вых. Данные | Год: 2015, Том: 119, Номер: 35, Страницы: 11466-11472 Страниц : 7 DOI: 10.1021/acs.jpcb.5b04160 | ||||||||
Ключевые слова | Activation energy; Binding sites; Cell membranes; Cytology; Kinetics; Refractive index; Viruses | ||||||||
Авторы |
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Организации |
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Информация о финансировании (4)
1 | Swedish Research Council | 2014-5557 |
2 | Swedish Research Council | 2012-5024 |
3 | Swedish Research Council | 2013-8266 |
4 | VINNOVA |
Реферат:
Many viruses interact with their host cells via glycosphingolipids (GSLs) and/or glycoproteins present on the outer cell membrane. This highly specific interaction includes virion attachment and detachment. The residence time determined by the detachment is particularly interesting, since it is directly related to internalization and infection as well as to virion egress and spreading. In an attempt to deepen the understanding of virion detachment kinetics, we have used total internal reflection fluorescence (TIRF) microscopy to probe the interaction between individual fluorescently labeled GSL-containing lipid vesicles and surface-bound virus-like particles (VLPs) of a norovirus genotype II.4 strain. The distribution of the VLP-vesicle residence time was investigated for seven naturally occurring GSLs, all of which are candidates for the not yet identified receptor(s) mediating norovirus entry into host cells. As expected for interactions involving multiple GSL binding sites at a viral capsid, the detachment kinetics displayed features typical for a broad activation-energy distribution for all GSLs. Detailed inspection of these distributions revealed significant differences among the different GSLs. The results are discussed in terms of strength of the interaction, vesicle size, as well as spatial distribution and clustering of GSLs in the vesicle membrane.
Библиографическая ссылка:
Nasir W.
, Bally M.
, Zhdanov V.P.
, Larson G.r.
, Höök F.
Interaction of Virus-Like Particles with Vesicles Containing Glycolipids: Kinetics of Detachment
The Journal of Physical Chemistry B. 2015. V.119. N35. P.11466-11472. DOI: 10.1021/acs.jpcb.5b04160 WOS Scopus РИНЦ
Interaction of Virus-Like Particles with Vesicles Containing Glycolipids: Kinetics of Detachment
The Journal of Physical Chemistry B. 2015. V.119. N35. P.11466-11472. DOI: 10.1021/acs.jpcb.5b04160 WOS Scopus РИНЦ
Даты:
Поступила в редакцию: | 30 апр. 2015 г. |
Принята к публикации: | 10 авг. 2015 г. |
Опубликована online: | 19 авг. 2015 г. |
Опубликована в печати: | 3 сент. 2015 г. |
Идентификаторы БД:
Web of science | WOS:000360947400003 |
Scopus | 2-s2.0-84941313212 |
РИНЦ | 24948784 |
Chemical Abstracts | 2015:1305479 |
Chemical Abstracts (print) | 163:317574 |
PMID (PubMed) | 26260011 |
OpenAlex | W1803480386 |