High-Performance Method for Specific Effect on Nucleic Acids in Cells Using TiO2~DNA Nanocomposites Full article
Journal |
Scientific Reports
, E-ISSN: 2045-2322 |
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Output data | Year: 2012, Volume: 2, Pages: 756 Pages count : 6 DOI: 10.1038/srep00756 | ||||||||
Tags | TITANIUM-DIOXIDE NANOPARTICLES; INFLUENZA-A VIRUS; ANTISENSE OLIGONUCLEOTIDES; RNA INTERFERENCE; DRUG-DELIVERY; THERAPEUTICS; REPLICATION; INHIBITION; TOXICITY; VECTORS | ||||||||
Authors |
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Affiliations |
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Funding (3)
1 | Russian Foundation for Basic Research | 11-04-01408 (01201158854) |
2 | The Ministry of Education and Science of the Russian Federation | 16.512.11.2267 (01201176480) |
3 | Siberian Branch of the Russian Academy of Sciences | 61 |
Abstract:
Nanoparticles are used to solve the current drug delivery problem. We present a high-performance method for efficient and selective action on nucleic acid target in cells using unique TiO2 center dot PL-DNA nanocomposites (polylysine-containing DNA fragments noncovalently immobilized onto TiO2 nanoparticles capable of transferring DNA). These nanocomposites were used for inhibition of human influenza A (H3N2) virus replication in infected MDCK cells. They showed a low toxicity (TC50 approximate to 1800 mu g/ml) and a high antiviral activity (>99.9% inhibition of the virus replication). The specificity factor (antisense effect) appeared to depend on the delivery system of DNA fragments. This factor for nanocomposites is ten-times higher than for DNA in the presence of lipofectamine. IC50 for nanocomposites was estimated to be 1.5 mu g/ml (30nM for DNA), so its selectivity index was calculated as similar to 1200. Thus, the proposed nanocomposites are prospective for therapeutic application.
Cite:
Levina A.S.
, Repkova M.N.
, Ismagilov Z.R.
, Shikina N.V.
, Malygin E.G.
, Mazurkova N.A.
, Zinovʹev V.V.
, Evdokimov A.A.
, Baiborodin S.I.
, Zarytova V.F.
High-Performance Method for Specific Effect on Nucleic Acids in Cells Using TiO2~DNA Nanocomposites
Scientific Reports. 2012. V.2. P.756. DOI: 10.1038/srep00756 WOS Scopus РИНЦ
High-Performance Method for Specific Effect on Nucleic Acids in Cells Using TiO2~DNA Nanocomposites
Scientific Reports. 2012. V.2. P.756. DOI: 10.1038/srep00756 WOS Scopus РИНЦ
Files:
Full text from publisher
Dates:
Submitted: | Jul 20, 2012 |
Accepted: | Sep 27, 2012 |
Published online: | Oct 22, 2012 |
Published print: | Dec 1, 2012 |
Identifiers:
Web of science | WOS:000310449200002 |
Scopus | 2-s2.0-84868297505 |
Elibrary | 20498444 |
Chemical Abstracts | 2012:1612669 |
Chemical Abstracts (print) | 159:416669 |
OpenAlex | W2086156791 |