Catalytic Properties of Glucoamylase Immobilized on Synthetic Carbon Material Sibunit Full article
| Journal |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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| Output data | Year: 2007, Volume: 43, Number: 4, Pages: 374-378 Pages count : 5 DOI: 10.1134/S0003683807040023 | ||||
| Tags | Apply Biochemistry; Immobilize Enzyme; Dextrin; Glucoamylase Activity; Mesoporous Carbon Material | ||||
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Abstract:
Glucoamylase (commercial preparation Glucavamorin) was immobilized by sorption on a carbon
support Sibunit. Starch saccharification by the resulting biocatalyst (dextrin hydrolysis) was studied. Investigation of the effect of adsorptional immobilization on kinetic parameters of glucoamylase, including the rate constant of thermal inactivation, showed that immobilization of Glucavamorin on Sibunit resulted in a thousandfold increase in glucoamylase stability in comparison with the dissolved enzyme. Presence of the substrate (dextrins) in the reaction mixture had a considerable stabilizing effect. Increase in dextrin concentration increases the thermostability of the immobilized enzyme. The overall factor of glucoamylase stabilization adsorbed on Sibunit with the presence of 53% dextrin solutions in comparison with the dissolved enzyme approximated 10 5. The biocatalyst for starch saccharification made on the base of Subunit-adsorbed Glucavamorin had a high operational stability. Its half-inactivation time at 60°ë exceeded 30 days.
Cite:
Kovalenko G.A.
, Perminova L.V.
, Terentʹeva T.G.
, Plaksin G.V.
Catalytic Properties of Glucoamylase Immobilized on Synthetic Carbon Material Sibunit
Applied Biochemistry and Microbiology. 2007. V.43. N4. P.374-378. DOI: 10.1134/S0003683807040023 WOS Scopus РИНЦ ANCAN OpenAlex
Catalytic Properties of Glucoamylase Immobilized on Synthetic Carbon Material Sibunit
Applied Biochemistry and Microbiology. 2007. V.43. N4. P.374-378. DOI: 10.1134/S0003683807040023 WOS Scopus РИНЦ ANCAN OpenAlex
Original:
Коваленко Г.А.
, Перминова Л.В.
, Терентьева Т.Г.
, Плаксин Г.В.
Каталитические свойства глюкоамилазы, иммобилизованной на углеродном носителе сибуните
Прикладная биохимия и микробиология. 2007. Т.43. №4. С.412-418. Scopus РИНЦ
Каталитические свойства глюкоамилазы, иммобилизованной на углеродном носителе сибуните
Прикладная биохимия и микробиология. 2007. Т.43. №4. С.412-418. Scopus РИНЦ
Dates:
| Submitted: | Oct 21, 2005 |
| Published print: | Jul 1, 2007 |
Identifiers:
| Web of science: | WOS:000248054600002 |
| Scopus: | 2-s2.0-34547184531 |
| Elibrary: | 13560532 |
| Chemical Abstracts: | 2007:768516 |
| Chemical Abstracts (print): | 147:421850 |
| OpenAlex: | W2061037713 |