Glucose Isomerase Activity in Suspensions of Arthrobacter nicotianae Cells and Adsorption Immobilization of the Microorganisms on Inorganic Carriers Full article
| Journal |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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| Output data | Year: 2008, Volume: 44, Number: 2, Pages: 174-181 Pages count : 8 DOI: 10.1134/S0003683808020087 | ||||||
| Tags | Fructose; Apply Biochemistry; Immobilize Cell; Foam Carbon; Foam Ceramic | ||||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | Belarusian Republican Foundation for Fundamental Research | Б07CO-011 |
| 2 | Siberian Branch of the Russian Academy of Sciences | 116044 |
Abstract:
Kinetics of monosaccharide isomerization has been studied in suspensions of intact, non-growing
Arthrobacter nicotianae cells. Under the conditions of the study, glucose and fructose were isomerized at the
same maximum rate of 700 µmol/min per 1 g dried cells, which increased with temperature (the dependence
was linear at 60–80°C). The proposed means of adsorption immobilization of A. nicotianae cells involve inorganic
carriers differing in macrostructure, chemical nature, and surface characteristics. Biocatalysts obtained
by adsorbing the cells of A. nicotianae on carbon-containing foamed ceramics in the coarse of submerged cultivation
were relatively stable and retained original activity (catalysis of monosaccharide isomerization)
throughout 14 h of use at 70°C. Maximum glucose isomerase activity (2 µmol/min per 1 g) was observed with
biocatalysts prepared by adsorption of non-growing A. nicotianae cells to the macroporous carbon–mineral carrier
Sapropel and subsequent drying of the cell suspension together with the carrier
Cite:
Kovalenko G.A.
, Perminova L.V.
, Terent’eva T.G.
, Sapunova L.I.
, Lobanok A.G.
, Chuenko T.V.
, Rudina N.A.
, Chernyak E.I.
Glucose Isomerase Activity in Suspensions of Arthrobacter nicotianae Cells and Adsorption Immobilization of the Microorganisms on Inorganic Carriers
Applied Biochemistry and Microbiology. 2008. V.44. N2. P.174-181. DOI: 10.1134/S0003683808020087 WOS Scopus РИНЦ ANCAN OpenAlex
Glucose Isomerase Activity in Suspensions of Arthrobacter nicotianae Cells and Adsorption Immobilization of the Microorganisms on Inorganic Carriers
Applied Biochemistry and Microbiology. 2008. V.44. N2. P.174-181. DOI: 10.1134/S0003683808020087 WOS Scopus РИНЦ ANCAN OpenAlex
Original:
Коваленко Г.А.
, Перминова Л.В.
, Терентьева Т.Г.
, Сапунова Л.И.
, Лобанок А.Г.
, Чуенко Т.В.
, Рудина Н.А.
, Черняк Е.И.
Активность глюкозоизомеразы в суспензии клеток Arthrobacter nicotianae и адсорбционная иммобилизация микроорганизмов на неорганических носителях
Прикладная биохимия и микробиология. 2008. Т.44. №2. С.193-201. Scopus РИНЦ
Активность глюкозоизомеразы в суспензии клеток Arthrobacter nicotianae и адсорбционная иммобилизация микроорганизмов на неорганических носителях
Прикладная биохимия и микробиология. 2008. Т.44. №2. С.193-201. Scopus РИНЦ
Dates:
| Submitted: | Jan 24, 2007 |
| Published print: | Mar 1, 2008 |
| Published online: | Jan 19, 2011 |
Identifiers:
| Web of science: | WOS:000254701900008 |
| Scopus: | 2-s2.0-41749101453 |
| Elibrary: | 13580714 |
| Chemical Abstracts: | 2008:429298 |
| Chemical Abstracts (print): | 149:550614 |
| OpenAlex: | W1988621400 |