Nanodiamond Bioconjugate Probes and Their Collection by Electrophoresis Научная публикация
| Журнал |
Diamond and Related Materials
ISSN: 0925-9635 , E-ISSN: 1879-0062 |
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| Вых. Данные | Год: 2008, Том: 17, Номер: 11, Страницы: 1858-1866 Страниц : 9 DOI: 10.1016/j.diamond.2008.03.020 | ||||
| Ключевые слова | Bioconjugation, Diamond, Electrophoresis, Nanotechnology, Streptavidin, Surface Chemistry | ||||
| Авторы |
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| Организации |
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Информация о финансировании (2)
| 1 | Defense Advanced Research Projects Agency | AFOSR FA9550-04-1-0440 |
| 2 | United States Army Research Laboratory | W911NF-04-2-0023 |
Реферат:
The application of detonation nanodiamonds (NDs) as probes for protein capture and electrophoretic collection was investigated. NDs were chemically modified in a series of reactions to produce a ND-NH2 product that had increased chemical homogeneity. The product was characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). FTIR spectra were taken using an IR vacuum cuvette and the samples were dehydrated at different temperatures. The ND-NH2 product was capable of conjugating to N-hydroxysuccinimide derivatives of TAMRA and biotin. We calculated that the number of chemically attached TAMRA molecules on ND-NH2 was ∼1 molecule/nm2. The singly conjugated TAMRA-ND (T-ND) and doubly conjugated TAMRA-ND-Biotin (T-ND-B) products formed stable aqueous colloidal suspensions. T-ND and T-ND-B were collected on planar electrodes and silicon field tip arrays using a field of 10 V/cm. The rate of collection for the aminated ND is dependent upon field strength and an exponential decrease in current was observed as a function of time. Streptavidin was captured by the T-ND-B bioconjugate probe and this nanoparticle–protein complex was collected from solution by electrophoresis.
Библиографическая ссылка:
Hens S.C.
, Cunningham G.
, Tyler T.
, Moseenkov S.
, Kuznetsov V.
, Shenderova O.
Nanodiamond Bioconjugate Probes and Their Collection by Electrophoresis
Diamond and Related Materials. 2008. V.17. N11. P.1858-1866. DOI: 10.1016/j.diamond.2008.03.020 WOS Scopus РИНЦ CAPlusCA OpenAlex
Nanodiamond Bioconjugate Probes and Their Collection by Electrophoresis
Diamond and Related Materials. 2008. V.17. N11. P.1858-1866. DOI: 10.1016/j.diamond.2008.03.020 WOS Scopus РИНЦ CAPlusCA OpenAlex
Даты:
| Поступила в редакцию: | 29 июн. 2007 г. |
| Принята к публикации: | 14 мар. 2008 г. |
| Опубликована online: | 1 апр. 2008 г. |
| Опубликована в печати: | 1 нояб. 2008 г. |
Идентификаторы БД:
| Web of science: | WOS:000260287600007 |
| Scopus: | 2-s2.0-52049090598 |
| РИНЦ: | 13589748 |
| Chemical Abstracts: | 2008:1137605 |
| Chemical Abstracts (print): | 150:92933 |
| OpenAlex: | W2083895468 |