Nanodiamond Bioconjugate Probes and Their Collection by Electrophoresis Full article
| Journal |
Diamond and Related Materials
ISSN: 0925-9635 , E-ISSN: 1879-0062 |
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| Output data | Year: 2008, Volume: 17, Number: 11, Pages: 1858-1866 Pages count : 9 DOI: 10.1016/j.diamond.2008.03.020 | ||||
| Tags | Bioconjugation, Diamond, Electrophoresis, Nanotechnology, Streptavidin, Surface Chemistry | ||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | Defense Advanced Research Projects Agency | AFOSR FA9550-04-1-0440 |
| 2 | United States Army Research Laboratory | W911NF-04-2-0023 |
Abstract:
The application of detonation nanodiamonds (NDs) as probes for protein capture and electrophoretic collection was investigated. NDs were chemically modified in a series of reactions to produce a ND-NH2 product that had increased chemical homogeneity. The product was characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). FTIR spectra were taken using an IR vacuum cuvette and the samples were dehydrated at different temperatures. The ND-NH2 product was capable of conjugating to N-hydroxysuccinimide derivatives of TAMRA and biotin. We calculated that the number of chemically attached TAMRA molecules on ND-NH2 was ∼1 molecule/nm2. The singly conjugated TAMRA-ND (T-ND) and doubly conjugated TAMRA-ND-Biotin (T-ND-B) products formed stable aqueous colloidal suspensions. T-ND and T-ND-B were collected on planar electrodes and silicon field tip arrays using a field of 10 V/cm. The rate of collection for the aminated ND is dependent upon field strength and an exponential decrease in current was observed as a function of time. Streptavidin was captured by the T-ND-B bioconjugate probe and this nanoparticle–protein complex was collected from solution by electrophoresis.
Cite:
Hens S.C.
, Cunningham G.
, Tyler T.
, Moseenkov S.
, Kuznetsov V.
, Shenderova O.
Nanodiamond Bioconjugate Probes and Their Collection by Electrophoresis
Diamond and Related Materials. 2008. V.17. N11. P.1858-1866. DOI: 10.1016/j.diamond.2008.03.020 WOS Scopus РИНЦ ANCAN OpenAlex
Nanodiamond Bioconjugate Probes and Their Collection by Electrophoresis
Diamond and Related Materials. 2008. V.17. N11. P.1858-1866. DOI: 10.1016/j.diamond.2008.03.020 WOS Scopus РИНЦ ANCAN OpenAlex
Dates:
| Submitted: | Jun 29, 2007 |
| Accepted: | Mar 14, 2008 |
| Published online: | Apr 1, 2008 |
| Published print: | Nov 1, 2008 |
Identifiers:
| Web of science: | WOS:000260287600007 |
| Scopus: | 2-s2.0-52049090598 |
| Elibrary: | 13589748 |
| Chemical Abstracts: | 2008:1137605 |
| Chemical Abstracts (print): | 150:92933 |
| OpenAlex: | W2083895468 |