Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor | Sciact - CRIS-система Института катализа

Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor Научная публикация

Журнал

Analytical Chemistry
ISSN: 0003-2700 , E-ISSN: 1520-6882

Вых. Данные

Год: 2017, Том: 89, Номер: 2, Страницы: 1102-1109 Страниц : 8 DOI: 10.1021/acs.analchem.6b02532

Ключевые слова

QUARTZ-CRYSTAL MICROBALANCE; LIPID-BILAYER FORMATION; SOLID-LIQUID INTERFACES; PHOSPHOLIPID-BILAYERS; ADSORPTION-KINETICS; VESICLE ADSORPTION; OSMOTIC-PRESSURE; MEMBRANES; SPECTROSCOPY; BIOSENSORS

Авторы

Jackman Joshua A. ^1,2 , Avsar Saziye Yorulmaz ^1,2 , Ferhan Abdul Rahim ^1,2 , Li Danlin ^1,2 , Park Jae Hyeon ^1,2 , Zhdanov Vladimir P. ^1,2,4 , Cho Nam-Joon ^1,2,3

Организации

1	School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Drive, 637553, Singapore
2	Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553, Singapore
3	School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Информация о финансировании (2)

1	Universiti Teknologi Nanyang
2	National Research Foundation of Singapore	NRF2015NRF-POC0001-019

Characterizing the shape of sub-100 nm, biological soft-matter particulates (e.g., liposomes and exosomes) adsorbed at a solid-liquid interface remains a challenging task. Here, we introduce a localized surface plasmon resonance (LSPR) sensing approach to quantitatively profile the deformation of nanoscale, fluid-phase 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes contacting a titanium dioxide substrate. Experimental and theoretical results validate that, due to its high sensitivity to the spatial proximity of phospholipid molecules near the sensor surface, the LSPR sensor can discriminate fine differences in the extent of ionic strength-modulated liposome deformation at both low and high surface coverages. By contrast, quartz crystal microbalance-dissipation (QCM-D) measurements performed with equivalent samples were qualitatively sensitive to liposome deformation only at saturation coverage. Control experiments with stiffer, gel-phase 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes verified that the LSPR measurement discrimination arises from the extent of liposome deformation, while the QCM-D measurements yield a more complex response that is also sensitive to the motion of adsorbed liposomes and coupled solvent along with lateral interactions between liposomes. Collectively, our findings demonstrate the unique measurement capabilities of LSPR sensors in the area of biological surface science, including competitive advantages for probing the shape properties of adsorbed, nanoscale biological particulates.

Jackman J.A. , Avsar S.Y. , Ferhan A.R. , Li D. , Park J.H. , Zhdanov V.P. , Cho N-J.
Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor
Analytical Chemistry. 2017. V.89. N2. P.1102-1109. DOI: 10.1021/acs.analchem.6b02532 WOS Scopus РИНЦ CAPlusCA PMID OpenAlex

Поступила в редакцию:	4 июл. 2016 г.
Принята к публикации:	16 дек. 2016 г.
Опубликована online:	28 дек. 2016 г.
Опубликована в печати:	17 янв. 2017 г.

Web of science:	WOS:000392458100013
Scopus:	2-s2.0-85021237145
РИНЦ:	31022740
Chemical Abstracts:	2016:2097150
Chemical Abstracts (print):	166:103047
PMID (PubMed):	27983791
OpenAlex:	W2567558047

БД	Цитирований
Web of science	54
Scopus	54
РИНЦ	52
OpenAlex	52