Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor | Sciact - CRIS-system of Boreskov Institute of Catalysis

Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor Full article

Journal

Analytical Chemistry
ISSN: 0003-2700 , E-ISSN: 1520-6882

Output data

Year: 2017, Volume: 89, Number: 2, Pages: 1102-1109 Pages count : 8 DOI: 10.1021/acs.analchem.6b02532

Tags

QUARTZ-CRYSTAL MICROBALANCE; LIPID-BILAYER FORMATION; SOLID-LIQUID INTERFACES; PHOSPHOLIPID-BILAYERS; ADSORPTION-KINETICS; VESICLE ADSORPTION; OSMOTIC-PRESSURE; MEMBRANES; SPECTROSCOPY; BIOSENSORS

Authors

Jackman Joshua A. ^1,2 , Avsar Saziye Yorulmaz ^1,2 , Ferhan Abdul Rahim ^1,2 , Li Danlin ^1,2 , Park Jae Hyeon ^1,2 , Zhdanov Vladimir P. ^1,2,4 , Cho Nam-Joon ^1,2,3

Affiliations

1	School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Drive, 637553, Singapore
2	Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553, Singapore
3	School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Funding (2)

1	Nanyang Technological University
2	National Research Foundation of Singapore	NRF2015NRF-POC0001-019

Characterizing the shape of sub-100 nm, biological soft-matter particulates (e.g., liposomes and exosomes) adsorbed at a solid-liquid interface remains a challenging task. Here, we introduce a localized surface plasmon resonance (LSPR) sensing approach to quantitatively profile the deformation of nanoscale, fluid-phase 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes contacting a titanium dioxide substrate. Experimental and theoretical results validate that, due to its high sensitivity to the spatial proximity of phospholipid molecules near the sensor surface, the LSPR sensor can discriminate fine differences in the extent of ionic strength-modulated liposome deformation at both low and high surface coverages. By contrast, quartz crystal microbalance-dissipation (QCM-D) measurements performed with equivalent samples were qualitatively sensitive to liposome deformation only at saturation coverage. Control experiments with stiffer, gel-phase 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes verified that the LSPR measurement discrimination arises from the extent of liposome deformation, while the QCM-D measurements yield a more complex response that is also sensitive to the motion of adsorbed liposomes and coupled solvent along with lateral interactions between liposomes. Collectively, our findings demonstrate the unique measurement capabilities of LSPR sensors in the area of biological surface science, including competitive advantages for probing the shape properties of adsorbed, nanoscale biological particulates.

Jackman J.A. , Avsar S.Y. , Ferhan A.R. , Li D. , Park J.H. , Zhdanov V.P. , Cho N-J.
Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor
Analytical Chemistry. 2017. V.89. N2. P.1102-1109. DOI: 10.1021/acs.analchem.6b02532 WOS Scopus РИНЦ ANCAN PMID OpenAlex

Submitted:	Jul 4, 2016
Accepted:	Dec 16, 2016
Published online:	Dec 28, 2016
Published print:	Jan 17, 2017

Web of science:	WOS:000392458100013
Scopus:	2-s2.0-85021237145
Elibrary:	31022740
Chemical Abstracts:	2016:2097150
Chemical Abstracts (print):	166:103047
PMID:	27983791
OpenAlex:	W2567558047

DB	Citing
Web of science	54
Scopus	54
Elibrary	53
OpenAlex	58