Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion | Sciact - CRIS-система Института катализа

Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion Научная публикация

Журнал

Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827

Вых. Данные

Год: 2017, Том: 33, Номер: 16, Страницы: 4049-4056 Страниц : 8 DOI: 10.1021/acs.langmuir.6b04582

Ключевые слова

Bins; Ligands; Lipid bilayers; Refractive index; Viruses

Авторы

Parveen Nagma ¹ , Block Stephan ¹ , Zhdanov Vladimir P. ^1,2 , Rydell Gustaf E. ³ , Höök Fredrik ¹

Организации

1	Department of Physics, Chalmers University of Technology, Gothenburg, Sweden
2	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk, Russia
3	Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

Информация о финансировании (4)

1	Swedish Research Council	2014-5557
2	Stiftelsen för strategisk forskning	RMA11-0104
3	Фонд Кнута и Элис Валленберг	2012.0055
4	Stiftelsen för strategisk forskning	SB12-0088

Multivalent receptor-mediated interactions between virions and a lipid membrane can be weakened using competitive nonpathogenic ligand binding. In particular, the subsequent binding of such ligands can induce detachment of bound virions, a phenomenon of crucial relevance for the development of new antiviral drugs. Focusing on the simian virus 40 (SV40) and recombinant cholera toxin B subunit (rCTB), and using (monosialotetrahexosyl)ganglioside (GM1) as their common receptor in a supported lipid bilayer (SLB), we present the first detailed investigation of this phenomenon by employing the quartz crystal microbalance with dissipation (QCM-D) and total internal reflection fluorescence (TIRF) microscopy assisted 2D single particle tracking (SPT) techniques. Analysis of the QCM-D-measured release kinetics made it possible to determine the binding strength of a single SV40–GM1 pair. The release dynamics of SV40, monitored by SPT, revealed that a notable fraction of SV40 becomes mobile just before the release, allowing to estimate the distribution of SV40-bound GM1 receptors just prior to release.

Parveen N. , Block S. , Zhdanov V.P. , Rydell G.E. , Höök F.
Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion

Langmuir. 2017. V.33. N16. P.4049-4056. DOI: 10.1021/acs.langmuir.6b04582 WOS Scopus РИНЦ РИНЦ CAPlusCA PMID OpenAlex

Полный текст от издателя

Поступила в редакцию:	21 дек. 2016 г.
Принята к публикации:	27 мар. 2017 г.
Опубликована online:	12 апр. 2017 г.
Опубликована в печати:	25 апр. 2017 г.

Web of science:	WOS:000400232500017
Scopus:	2-s2.0-85018630559
РИНЦ:	29509627 \| 41773555
Chemical Abstracts:	2017:500947
Chemical Abstracts (print):	166:392332
PMID (PubMed):	28350474
OpenAlex:	W2599768242

БД	Цитирований
Web of science	16
Scopus	17
РИНЦ	4 14
OpenAlex	17