Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion Full article
| Journal |
Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827 |
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| Output data | Year: 2017, Volume: 33, Number: 16, Pages: 4049-4056 Pages count : 8 DOI: 10.1021/acs.langmuir.6b04582 | ||||||
| Tags | Bins; Ligands; Lipid bilayers; Refractive index; Viruses | ||||||
| Authors |
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| Affiliations |
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Funding (4)
| 1 | Swedish Research Council | 2014-5557 |
| 2 | Stiftelsen för strategisk forskning | RMA11-0104 |
| 3 | Knut and Alice Wallenberg Foundation | 2012.0055 |
| 4 | Stiftelsen för strategisk forskning | SB12-0088 |
Abstract:
Multivalent receptor-mediated interactions between virions and a lipid membrane can be weakened using competitive nonpathogenic ligand binding. In particular, the subsequent binding of such ligands can induce detachment of bound virions, a phenomenon of crucial relevance for the development of new antiviral drugs. Focusing on the simian virus 40 (SV40) and recombinant cholera toxin B subunit (rCTB), and using (monosialotetrahexosyl)ganglioside (GM1) as their common receptor in a supported lipid bilayer (SLB), we present the first detailed investigation of this phenomenon by employing the quartz crystal microbalance with dissipation (QCM-D) and total internal reflection fluorescence (TIRF) microscopy assisted 2D single particle tracking (SPT) techniques. Analysis of the QCM-D-measured release kinetics made it possible to determine the binding strength of a single SV40–GM1 pair. The release dynamics of SV40, monitored by SPT, revealed that a notable fraction of SV40 becomes mobile just before the release, allowing to estimate the distribution of SV40-bound GM1 receptors just prior to release.
Cite:
Parveen N.
, Block S.
, Zhdanov V.P.
, Rydell G.E.
, Höök F.
Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion
Langmuir. 2017.
V.33. N16. P.4049-4056. DOI: 10.1021/acs.langmuir.6b04582
WOS
Scopus
РИНЦ
РИНЦ
ANCAN
PMID
OpenAlex
Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion
Langmuir. 2017.
V.33. N16. P.4049-4056. DOI: 10.1021/acs.langmuir.6b04582
WOS
Scopus
РИНЦ
РИНЦ
ANCAN
PMID
OpenAlex
Files:
Full text from publisher
Dates:
| Submitted: | Dec 21, 2016 |
| Accepted: | Mar 27, 2017 |
| Published online: | Apr 12, 2017 |
| Published print: | Apr 25, 2017 |
Identifiers:
| Web of science: | WOS:000400232500017 |
| Scopus: | 2-s2.0-85018630559 |
| Elibrary: | 29509627 | 41773555 |
| Chemical Abstracts: | 2017:500947 |
| Chemical Abstracts (print): | 166:392332 |
| PMID: | 28350474 |
| OpenAlex: | W2599768242 |