Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic | Sciact - CRIS-система Института катализа

Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic Научная публикация

Журнал

Journal of Physical Chemistry Letters
ISSN: 1948-7185

Вых. Данные

Год: 2018, Том: 9, Страницы: 2278-2284 Страниц : 7 DOI: 10.1021/acs.jpclett.8b00433

Ключевые слова

BLOOD-GROUP ANTIGENS; LIPID RAFTS; PROTEINS; BINDING; VIRUS; INVAGINATIONS; VLP

Авторы

Parveen Nagma ^1,5 , Rimkute Inga ² , Block Stephan ^1,6 , Rydell Gustaf E. ² , Midtvedt Daniel ¹ , Larson Göran ² , Hytönen Vesa P. ³ , Zhdanov Vladimir P. ^1,4 , Lundgren Anders ¹ , Höök Fredrik ^1,7

Организации

1	Department of Physics, Chalmers University of Technology, Gothenburg, Sweden
2	Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
3	Faculty of Medicine and Life Sciences and BioMediTech, University of Tampere, Finland and Fimlab Laboratories, Tampere, Finland
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk, Russia
5	Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, KU Leuven, Leuven, Belgium
6	Department of Chemistry and Biochemistry, Free University of Berlin, Berlin, Germany
7	Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden

Информация о финансировании (3)

1	Swedish Research Council	2013-7421
2	Swedish Research Council	2014-5557
3	Stiftelsen för strategisk forskning	RMA11-0104

Quartz crystal microbalance with dissipation monitoring and total internal reflection fluorescence microscopy have been used to investigate binding of norovirus-like particles (noroVLPs) to a supported (phospho)lipid bilayer (SLB) containing a few percent of H or B type 1 glycosphingolipid (GSL) receptors. Although neither of these GSLs spontaneously form domains, noroVLPs were observed to form micron-sized clusters containing typically up to about 30 VLP copies, especially for B type 1, which is a higher-affinity receptor. This novel finding is explained by proposing a model implying that VLP-induced membrane deformation promotes VLP clustering, a hypothesis that was further supported by observing that functionalized gold nanoparticles were able to locally induce SLB deformation. Because similar effects are likely possible also at cellular membranes, our findings are interesting beyond a pure biophysicochemical perspective as they shed new light on what may happen during receptor-mediated uptake of viruses as well as nanocarriers in drug delivery.

Parveen N. , Rimkute I. , Block S. , Rydell G.E. , Midtvedt D. , Larson G. , Hytönen V.P. , Zhdanov V.P. , Lundgren A. , Höök F.
Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic

Journal of Physical Chemistry Letters. 2018. V.9. P.2278-2284. DOI: 10.1021/acs.jpclett.8b00433 WOS Scopus РИНЦ CAPlus PMID OpenAlex

Полный текст от издателя

Поступила в редакцию:	9 февр. 2018 г.
Принята к публикации:	6 апр. 2018 г.
Опубликована online:	6 апр. 2018 г.
Опубликована в печати:	3 мая 2018 г.

Web of science:	WOS:000431724400028
Scopus:	2-s2.0-85046533287
РИНЦ:	35493261
Chemical Abstracts:	2018:722489
PMID (PubMed):	29624391
OpenAlex:	W2796254055

БД	Цитирований
Web of science	12
Scopus	12
РИНЦ	10
OpenAlex	12