Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic | Sciact - CRIS-system of Boreskov Institute of Catalysis

Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic Full article

Journal

Journal of Physical Chemistry Letters
ISSN: 1948-7185

Output data

Year: 2018, Volume: 9, Pages: 2278-2284 Pages count : 7 DOI: 10.1021/acs.jpclett.8b00433

Tags

BLOOD-GROUP ANTIGENS; LIPID RAFTS; PROTEINS; BINDING; VIRUS; INVAGINATIONS; VLP

Authors

Parveen Nagma ^1,5 , Rimkute Inga ² , Block Stephan ^1,6 , Rydell Gustaf E. ² , Midtvedt Daniel ¹ , Larson Göran ² , Hytönen Vesa P. ³ , Zhdanov Vladimir P. ^1,4 , Lundgren Anders ¹ , Höök Fredrik ^1,7

Affiliations

1	Department of Physics, Chalmers University of Technology, Gothenburg, Sweden
2	Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
3	Faculty of Medicine and Life Sciences and BioMediTech, University of Tampere, Finland and Fimlab Laboratories, Tampere, Finland
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk, Russia
5	Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, KU Leuven, Leuven, Belgium
6	Department of Chemistry and Biochemistry, Free University of Berlin, Berlin, Germany
7	Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden

Funding (3)

1	Swedish Research Council	2013-7421
2	Swedish Research Council	2014-5557
3	Stiftelsen för strategisk forskning	RMA11-0104

Quartz crystal microbalance with dissipation monitoring and total internal reflection fluorescence microscopy have been used to investigate binding of norovirus-like particles (noroVLPs) to a supported (phospho)lipid bilayer (SLB) containing a few percent of H or B type 1 glycosphingolipid (GSL) receptors. Although neither of these GSLs spontaneously form domains, noroVLPs were observed to form micron-sized clusters containing typically up to about 30 VLP copies, especially for B type 1, which is a higher-affinity receptor. This novel finding is explained by proposing a model implying that VLP-induced membrane deformation promotes VLP clustering, a hypothesis that was further supported by observing that functionalized gold nanoparticles were able to locally induce SLB deformation. Because similar effects are likely possible also at cellular membranes, our findings are interesting beyond a pure biophysicochemical perspective as they shed new light on what may happen during receptor-mediated uptake of viruses as well as nanocarriers in drug delivery.

Parveen N. , Rimkute I. , Block S. , Rydell G.E. , Midtvedt D. , Larson G. , Hytönen V.P. , Zhdanov V.P. , Lundgren A. , Höök F.
Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic

Journal of Physical Chemistry Letters. 2018. V.9. P.2278-2284. DOI: 10.1021/acs.jpclett.8b00433 WOS Scopus РИНЦ AN PMID OpenAlex

Full text from publisher

Submitted:	Feb 9, 2018
Accepted:	Apr 6, 2018
Published online:	Apr 6, 2018
Published print:	May 3, 2018

Web of science:	WOS:000431724400028
Scopus:	2-s2.0-85046533287
Elibrary:	35493261
Chemical Abstracts:	2018:722489
PMID:	29624391
OpenAlex:	W2796254055

DB	Citing
Web of science	12
Scopus	12
Elibrary	10
OpenAlex	12