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Mechanical Properties and Biological Behavior of 3D Matrices Produced by Electrospinning from Protein-Enriched Polyurethane Full article

Journal BioMed Research International
ISSN: 2314-6133 , E-ISSN: 2314-6141
Output data Year: 2018, Volume: 2018, Article number : 1380606, Pages count : 10 DOI: 10.1155/2018/1380606
Tags HUMAN ENDOTHELIAL-CELLS; TISSUE ENGINEERING APPLICATIONS; VASCULAR GRAFT; TECOPHILIC/GELATIN NANOFIBERS; SURFACE-ROUGHNESS; IN-VITRO; SCAFFOLDS; GELATIN; DEGRADATION; RELEASE
Authors Chernonosova Vera S. 1,2 , Gostev Alexander A. 2 , Gao Yun 3 , Chesalov Yuriy A. 4 , Shutov Alexey V. 5 , Pokushalov Evgeniy A. 2 , Karpenko Andrey A. 2 , Laktionov Pavel P. 1,2
Affiliations
1 Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, Russia
2 Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk 630055, Russia
3 Novosibirsk State University, Novosibirsk 630090, Russia
4 Boreskov Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia
5 Lavrentyev Institute of Hydrodynamics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

Funding (1)

1 Russian Science Foundation 17-75-30009

Abstract: Properties of matrices manufactured by electrospinning from solutions of polyurethane Tecoflex EG-80A with gelatin in 1,1,1,3,3,3-hexafluoroisopropanol were studied. The concentration of gelatin added to the electrospinning solution was shown to influence the mechanical properties of matrices: the dependence of matrix tensile strength on protein concentration is described by a bell-shaped curve and an increase in gelatin concentration added to the elasticity of the samples. SEM, FTIR spectroscopy, and mechanical testing demonstrate that incubation of matrices in phosphate buffer changes the structure of the fibers and alters the polyurethane-gelatin interactions, increasing matrix durability. The ability of the matrices to maintain adhesion and proliferation of human endothelial cells was studied. The results suggest that matrices made of 3% polyurethane solution with 15% gelatin (wt/wt) and treated with glutaraldehyde are the optimal variant for cultivation of endothelial cells.
Cite: Chernonosova V.S. , Gostev A.A. , Gao Y. , Chesalov Y.A. , Shutov A.V. , Pokushalov E.A. , Karpenko A.A. , Laktionov P.P.
Mechanical Properties and Biological Behavior of 3D Matrices Produced by Electrospinning from Protein-Enriched Polyurethane
BioMed Research International. 2018. V.2018. 1380606 :1-10. DOI: 10.1155/2018/1380606 WOS Scopus РИНЦ AN PMID OpenAlex
Files: Full text from publisher
Dates:
Submitted: Mar 6, 2018
Accepted: May 29, 2018
Published online: Jun 26, 2018
Published print: Jul 26, 2018
Identifiers:
Web of science: WOS:000436187300001
Scopus: 2-s2.0-85049830964
Elibrary: 35777178
Chemical Abstracts: 2018:2263778
PMID: 30046587
OpenAlex: W2809826731
Citing:
DB Citing
Web of science 23
Scopus 24
Elibrary 23
OpenAlex 25
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