Spatiotemporal Kinetics of Supported Lipid Bilayer Formation on Glass via Vesicle Adsorption and Rupture Научная публикация
| Журнал |
Journal of Physical Chemistry Letters
ISSN: 1948-7185 |
||||
|---|---|---|---|---|---|
| Вых. Данные | Год: 2018, Том: 9, Номер: 17, Страницы: 5143-5149 Страниц : 7 DOI: 10.1021/acs.jpclett.8b02092 | ||||
| Ключевые слова | Cytology Fluorescence microscopy Intelligent systems Lipid bilayers Monte Carlo methods Refractive index Silica | ||||
| Авторы |
|
||||
| Организации |
|
Информация о финансировании (2)
| 1 | Swedish Research Council | 2014-5557 |
| 2 | Stiftelsen för strategisk forskning | RMA11-0104 |
Реферат:
Supported lipid bilayers (SLBs) represent one of the most popular mimics of the cell membrane. Herein, we have used total internal reflection fluorescence microscopy for in-depth characterization of the vesicle-mediated SLB formation mechanism on a common silica-rich substrate, borosilicate glass. Fluorescently labeling a subset of vesicles allowed us to monitor the adsorption of individual labeled vesicles, resolve the onset of SLB formation from small seeds of SLB patches, and track their growth via SLB-edge-induced autocatalytic rupture of adsorbed vesicles. This made it possible to perform the first quantitative measurement of the SLB front velocity, which is shown to increase up to 1 order of magnitude with time. This effect can be classified as dramatic because in many other physical, chemical, or biological kinetic processes the front velocity is either constant or decreasing with time. The observation was successfully described with a theoretical model and Monte Carlo simulations implying rapid local diffusion of lipids upon vesicle rupture.
Библиографическая ссылка:
Mapar M.
, Jõemetsa S.
, Pace H.
, Zhdanov V.P.
, Agnarsson B.
, Höök F.
Spatiotemporal Kinetics of Supported Lipid Bilayer Formation on Glass via Vesicle Adsorption and Rupture
Journal of Physical Chemistry Letters. 2018. V.9. N17. P.5143-5149. DOI: 10.1021/acs.jpclett.8b02092 WOS Scopus РИНЦ CAPlus PMID OpenAlex
Spatiotemporal Kinetics of Supported Lipid Bilayer Formation on Glass via Vesicle Adsorption and Rupture
Journal of Physical Chemistry Letters. 2018. V.9. N17. P.5143-5149. DOI: 10.1021/acs.jpclett.8b02092 WOS Scopus РИНЦ CAPlus PMID OpenAlex
Даты:
| Поступила в редакцию: | 4 июл. 2018 г. |
| Принята к публикации: | 23 авг. 2018 г. |
| Опубликована online: | 23 авг. 2018 г. |
| Опубликована в печати: | 6 сент. 2018 г. |
Идентификаторы БД:
| Web of science: | WOS:000444353900051 |
| Scopus: | 2-s2.0-85052919155 |
| РИНЦ: | 35720997 |
| Chemical Abstracts: | 2018:1576379 |
| PMID (PubMed): | 30137991 |
| OpenAlex: | W2888667343 |