Spatiotemporal Kinetics of Supported Lipid Bilayer Formation on Glass via Vesicle Adsorption and Rupture Full article
| Journal |
Journal of Physical Chemistry Letters
ISSN: 1948-7185 |
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| Output data | Year: 2018, Volume: 9, Number: 17, Pages: 5143-5149 Pages count : 7 DOI: 10.1021/acs.jpclett.8b02092 | ||||
| Tags | Cytology Fluorescence microscopy Intelligent systems Lipid bilayers Monte Carlo methods Refractive index Silica | ||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | Swedish Research Council | 2014-5557 |
| 2 | Stiftelsen för strategisk forskning | RMA11-0104 |
Abstract:
Supported lipid bilayers (SLBs) represent one of the most popular mimics of the cell membrane. Herein, we have used total internal reflection fluorescence microscopy for in-depth characterization of the vesicle-mediated SLB formation mechanism on a common silica-rich substrate, borosilicate glass. Fluorescently labeling a subset of vesicles allowed us to monitor the adsorption of individual labeled vesicles, resolve the onset of SLB formation from small seeds of SLB patches, and track their growth via SLB-edge-induced autocatalytic rupture of adsorbed vesicles. This made it possible to perform the first quantitative measurement of the SLB front velocity, which is shown to increase up to 1 order of magnitude with time. This effect can be classified as dramatic because in many other physical, chemical, or biological kinetic processes the front velocity is either constant or decreasing with time. The observation was successfully described with a theoretical model and Monte Carlo simulations implying rapid local diffusion of lipids upon vesicle rupture.
Cite:
Mapar M.
, Jõemetsa S.
, Pace H.
, Zhdanov V.P.
, Agnarsson B.
, Höök F.
Spatiotemporal Kinetics of Supported Lipid Bilayer Formation on Glass via Vesicle Adsorption and Rupture
Journal of Physical Chemistry Letters. 2018. V.9. N17. P.5143-5149. DOI: 10.1021/acs.jpclett.8b02092 WOS Scopus РИНЦ AN PMID OpenAlex
Spatiotemporal Kinetics of Supported Lipid Bilayer Formation on Glass via Vesicle Adsorption and Rupture
Journal of Physical Chemistry Letters. 2018. V.9. N17. P.5143-5149. DOI: 10.1021/acs.jpclett.8b02092 WOS Scopus РИНЦ AN PMID OpenAlex
Dates:
| Submitted: | Jul 4, 2018 |
| Accepted: | Aug 23, 2018 |
| Published online: | Aug 23, 2018 |
| Published print: | Sep 6, 2018 |
Identifiers:
| Web of science: | WOS:000444353900051 |
| Scopus: | 2-s2.0-85052919155 |
| Elibrary: | 35720997 |
| Chemical Abstracts: | 2018:1576379 |
| PMID: | 30137991 |
| OpenAlex: | W2888667343 |