Bioprospecting Thermophilic Glycosyl Hydrolases, from Hot Springs of Himachal Pradesh, for Biomass Valorization | Sciact - CRIS-система Института катализа

Bioprospecting Thermophilic Glycosyl Hydrolases, from Hot Springs of Himachal Pradesh, for Biomass Valorization Научная публикация

Журнал

AMB Express
, E-ISSN: 2191-0855

Вых. Данные

Год: 2018, Том: 8, Номер статьи : 168, Страниц : 15 DOI: 10.1186/s13568-018-0690-4

Ключевые слова

Glycosyl hydrolases, Thermophilic, Biomass, Hot springs

Авторы

Thankappan Sugitha ¹ , Kandasamy Sujatha ¹ , Joshi Beslin ¹ , Sorokina Ksenia N. ² , Taran Oxana P. ² , Uthandi Sivakumar ¹

Организации

1	Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641003, India
2	Boreskov Institute of Catalysis (BIC), Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia

Информация о финансировании (1)

Department of Biotechnology

DBT/IC-2/Indo-Russia/2014-16/04

The harnessing of biocatalysts from extreme environment hot spring niche for biomass conversion is significant and promising owing to the special characteristics of extremozymes attributed by intriguing biogeochemistry and extreme conditions of these environments. Hence, in the present study 38 bacterial isolates obtained from hot springs of Manikaran (~ 95 °C), Kalath (~ 50 °C) and Vasist (~ 65 °C) of Himachal Pradesh were screened for glycosyl hydrolases by in situ enrichment technique using lignocellulosic biomass (LCB). Based on their hydrolytic potential 5 isolates were selected and they were Bacillus tequilensis (VCB1, VCB2 and VSDB4), and B. licheniformis (KBFB2 and KBFB3). Cellulolytic activity assayed by growth under submerged fermentation showed that B. tequilensis VCB1 had maximum FPA activity (3.38 IU ml−1) in 48 h, while B. licheniformis KBFB3 excelled for endoglucanase (EGA of 4.81 IU ml−1 in 24 h) and cellobiase (0.71 IU ml−1 in 48 h) activities. Among all the thermophilic biocatalysts evaluated, highest exoglucanase (0.06 IU ml−1) activity was observed in B. tequilensis VSDB4 while endoglucanase of B. licheniformis KBFB3 showed optimum specific activity at pH 7 and 70 °C. Further, the presence of celS, celB and xlnB genes in the isolates suggest their possible role in biomass conversion. Protein profiling by SDS-PAGE analysis revealed that cellulase isoforms migrated with molecular masses of 75 kDa. The endoglucanase activity of promising strain B. licheniformis KBFB3 was enhanced in the presence of Ca2+, mercaptoethanol and sodium hypochlorite whereas moderately inhibited by Cu2+, Zn2+, urea, SDS and H2O2. The results of this study indicate scope for the possible development of novel biocatalysts with multifunctional thermostable glycosyl hydrolases from hot springs for efficient hydrolysis of the complex lignocellulosic biomass into simple sugars and other derived bioproducts leading to biomass valorization.

Thankappan S. , Kandasamy S. , Joshi B. , Sorokina K.N. , Taran O.P. , Uthandi S.
Bioprospecting Thermophilic Glycosyl Hydrolases, from Hot Springs of Himachal Pradesh, for Biomass Valorization

AMB Express. 2018. V.8. 168 :1-15. DOI: 10.1186/s13568-018-0690-4 WOS Scopus РИНЦ CAPlus PMID OpenAlex

Полный текст от издателя

Поступила в редакцию:	6 июн. 2018 г.
Принята к публикации:	26 сент. 2018 г.
Опубликована online:	15 окт. 2018 г.
Опубликована в печати:	1 дек. 2018 г.

Web of science:	WOS:000447488900001
Scopus:	2-s2.0-85055041982
РИНЦ:	38617409
Chemical Abstracts:	2018:1960169
PMID (PubMed):	30324223
OpenAlex:	W2897638542

БД	Цитирований
Web of science	9
Scopus	12
РИНЦ	10
OpenAlex	14