The Lipid Metabolites of Blood Serum and Erythrocytes: The Opportunities for Diagnosis of Colorectal Cancer | Sciact - CRIS-система Института катализа

The Lipid Metabolites of Blood Serum and Erythrocytes: The Opportunities for Diagnosis of Colorectal Cancer Тезисы доклада

Конференция

8th European Conference on Predictive, Preventive & Personalized Medicine and Molecular Diagnostics
20-21 авг. 2018 , Rome

Сборник

Proceedings of 8th European Conference on Predictive, Preventive and Personalized Medicine&molecular Diagnostics
Сборник, Conference Series IIc LTD. UK.2018. 91 c.

Вых. Данные

Год: 2018, Страницы: 64 Страниц : 1

Ключевые слова

lipid metabolites,blood serum,erythrocyte,diagnosis,colorectal cancer

Авторы

Kruchinina M.V. ¹ , Gromov A.A. ¹ , Shashkov M.V. ² , Sokolova A.S. ³ , Kruchinin V.N. ⁴ , Prudnikova Ya.I. ¹

Организации

1	Institute of Cytology and Genetics-SB RAS, Russia
2	Boreskov Institute of Catalysis-SB RAS, Russia
3	N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry-SB RAS, Russia
4	Rzhanov’s Institute of Semiconductor Physics-SB RAS, Russia

The aim of the work was to evaluate lipid metabolites of blood serum (BS) and red blood cells (RBCs) as potential biomarkers for the diagnosis of colorectal cancer (CRC). BS, RBCs from 95 patients (56+8 years old) with colorectal adenocarcinoma (early stages I-II, n = 44; late stages III-IV, n=51) and 28 healthy controls were analyzed by FT mass spectrometry, GC/MS system triple quad Agilent 7000B(USA). The list 1 of the BS lipid metabolites - amines of fatty acids (FA) (palmitic amide, erucamide, oleamide), saturated FA (C14:0, 16:0, 18:0), polyunsaturated FA (C20:3, 20:4), lysophosphatidylcholines (m>2) (C18:2, 20:2, 20:4, 20:6, 22:6) allowed us to obtain the high level of AUC 0.98 (sensitivity 0,97, specificity 0,99) when comparing early stage CRC patients with controls. Panel 2 including the following lipids of BS - palmitic amide, erucamide, oleamide, miristic, palmitic, stearic acids, lysophosphatidic acids, LPC(16:0)- showed good opportunities for distinguishing the CRC patients from healthy controls (AUC 0,95 (0,8, 0,96). The combination of the lipids - phosphatidylcholine, phosphatidylserine, lysophosphatidylcholines (18:0, 18:1, 18:2, 20:4, 20:6, 22:6), palmitic acid – differentiated the late stages from the early ones (AUC 0,91 (0,80, 0,92). Differentiating FA of RBCs membranes were polyunsaturated (C20:0, C20:2, C20:3, C20:4, C22:4, C22:5, C22:6), the levels of which were significantly higher in CRC patients, and saturated and monounsaturated (C 12-17:0, C16:1, C 18:1, C18:2), prevailing in healthy controls. The observed shifts correlated with the CRC stages. The lipid metabolites of blood serum and erythrocytes should be considered as the promising biomarkers for CRC diagnosis.

Kruchinina M.V. , Gromov A.A. , Shashkov M.V. , Sokolova A.S. , Kruchinin V.N. , Prudnikova Y.I.
The Lipid Metabolites of Blood Serum and Erythrocytes: The Opportunities for Diagnosis of Colorectal Cancer
В сборнике Proceedings of 8th European Conference on Predictive, Preventive and Personalized Medicine&molecular Diagnostics. – Conference Series IIc LTD., 2018. – C.64. РИНЦ

РИНЦ:

35566100

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