The Lipid Metabolites of Blood Serum and Erythrocytes: The Opportunities for Diagnosis of Colorectal Cancer | Sciact - CRIS-system of Boreskov Institute of Catalysis

The Lipid Metabolites of Blood Serum and Erythrocytes: The Opportunities for Diagnosis of Colorectal Cancer Conference Abstracts

Conference

8th European Conference on Predictive, Preventive & Personalized Medicine and Molecular Diagnostics
20-21 Aug 2018 , Rome

Source

Proceedings of 8th European Conference on Predictive, Preventive and Personalized Medicine&molecular Diagnostics
Compilation, Conference Series IIc LTD. UK.2018. 91 c.

Output data

Year: 2018, Pages: 64 Pages count : 1

Tags

lipid metabolites,blood serum,erythrocyte,diagnosis,colorectal cancer

Authors

Kruchinina M.V. ¹ , Gromov A.A. ¹ , Shashkov M.V. ² , Sokolova A.S. ³ , Kruchinin V.N. ⁴ , Prudnikova Ya.I. ¹

Affiliations

1	Institute of Cytology and Genetics-SB RAS, Russia
2	Boreskov Institute of Catalysis-SB RAS, Russia
3	N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry-SB RAS, Russia
4	Rzhanov’s Institute of Semiconductor Physics-SB RAS, Russia

The aim of the work was to evaluate lipid metabolites of blood serum (BS) and red blood cells (RBCs) as potential biomarkers for the diagnosis of colorectal cancer (CRC). BS, RBCs from 95 patients (56+8 years old) with colorectal adenocarcinoma (early stages I-II, n = 44; late stages III-IV, n=51) and 28 healthy controls were analyzed by FT mass spectrometry, GC/MS system triple quad Agilent 7000B(USA). The list 1 of the BS lipid metabolites - amines of fatty acids (FA) (palmitic amide, erucamide, oleamide), saturated FA (C14:0, 16:0, 18:0), polyunsaturated FA (C20:3, 20:4), lysophosphatidylcholines (m>2) (C18:2, 20:2, 20:4, 20:6, 22:6) allowed us to obtain the high level of AUC 0.98 (sensitivity 0,97, specificity 0,99) when comparing early stage CRC patients with controls. Panel 2 including the following lipids of BS - palmitic amide, erucamide, oleamide, miristic, palmitic, stearic acids, lysophosphatidic acids, LPC(16:0)- showed good opportunities for distinguishing the CRC patients from healthy controls (AUC 0,95 (0,8, 0,96). The combination of the lipids - phosphatidylcholine, phosphatidylserine, lysophosphatidylcholines (18:0, 18:1, 18:2, 20:4, 20:6, 22:6), palmitic acid – differentiated the late stages from the early ones (AUC 0,91 (0,80, 0,92). Differentiating FA of RBCs membranes were polyunsaturated (C20:0, C20:2, C20:3, C20:4, C22:4, C22:5, C22:6), the levels of which were significantly higher in CRC patients, and saturated and monounsaturated (C 12-17:0, C16:1, C 18:1, C18:2), prevailing in healthy controls. The observed shifts correlated with the CRC stages. The lipid metabolites of blood serum and erythrocytes should be considered as the promising biomarkers for CRC diagnosis.

Kruchinina M.V. , Gromov A.A. , Shashkov M.V. , Sokolova A.S. , Kruchinin V.N. , Prudnikova Y.I.
The Lipid Metabolites of Blood Serum and Erythrocytes: The Opportunities for Diagnosis of Colorectal Cancer
In compilation Proceedings of 8th European Conference on Predictive, Preventive and Personalized Medicine&molecular Diagnostics. – Conference Series IIc LTD., 2018. – C.64. РИНЦ

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