Real-Time Nanoplasmonic Sensing of Three-Dimensional Morphological Changes in a Supported Lipid Bilayer and Antimicrobial Testing Applications Научная публикация
| Журнал |
Biosensors and Bioelectronics
ISSN: 0956-5663 , E-ISSN: 1873-4235 |
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| Вых. Данные | Год: 2021, Том: 174, Номер статьи : 112768, Страниц : 8 DOI: 10.1016/j.bios.2020.112768 | ||||||
| Ключевые слова | nanoplasmonics; localized surface plasmon resonance; label-free biosensing; artificial cell membrane; antimicrobia | ||||||
| Авторы |
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| Организации |
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Информация о финансировании (2)
| 1 | National Research Foundation of Korea | 2020R1C1C1004385 |
| 2 | National Research Foundation of Korea | 2019H1D3A1A01070318 |
Реферат:
We report the development of a real-time localized surface plasmon resonance (LSPR) biosensing strategy to detect three-dimensional morphological changes in a supported lipid bilayer (SLB) on a plasmonic substrate. The sensing concept advances on past efforts to detect subtle conformational changes in adsorbed biomacromolecules by demonstrating the capability to track large-scale, complex adsorbate shape changes and to classify different types of shape changes based on specific, multi-step measurement signatures. To validate this concept, we tested the addition of antimicrobial fatty acids, monoglycerides, and surfactants in micellar form to the SLB platform, which triggered specific three-dimensional membrane morphological changes such as tubule or bud formation along with solubilization. Experimentally, the different remodeling events were detected by distinct measurement signatures related to the shape and size of lipid protrusions that formed and evolved over time, which agreed well with a newly developed theoretical model. Our conceptual approach and formalism are applicable to various biosensing techniques, including not only LSPR but also surface plasmon resonance (SPR) and total internal reflection fluorescence (TIRF) microscopy. These sensing capabilities are advantageous for evaluating the mechanisms of antimicrobial drug candidates and other membrane-active compounds, and the measurement strategy is extendable to a wide range of biomimetic lipid compositions.
Библиографическая ссылка:
Yoon B.K.
, Park H.
, Zhdanov V.P.
, Jackman J.A.
, Cho N-J.
Real-Time Nanoplasmonic Sensing of Three-Dimensional Morphological Changes in a Supported Lipid Bilayer and Antimicrobial Testing Applications
Biosensors and Bioelectronics. 2021. V.174. 112768 :1-8. DOI: 10.1016/j.bios.2020.112768 WOS Scopus РИНЦ CAPlus PMID OpenAlex
Real-Time Nanoplasmonic Sensing of Three-Dimensional Morphological Changes in a Supported Lipid Bilayer and Antimicrobial Testing Applications
Biosensors and Bioelectronics. 2021. V.174. 112768 :1-8. DOI: 10.1016/j.bios.2020.112768 WOS Scopus РИНЦ CAPlus PMID OpenAlex
Даты:
| Поступила в редакцию: | 6 авг. 2020 г. |
| Принята к публикации: | 23 окт. 2020 г. |
| Опубликована online: | 31 окт. 2020 г. |
| Опубликована в печати: | 15 февр. 2021 г. |
Идентификаторы БД:
| Web of science: | WOS:000605488400009 |
| Scopus: | 2-s2.0-85097229358 |
| РИНЦ: | 45100230 |
| Chemical Abstracts: | 2020:2517680 |
| PMID (PubMed): | 33288427 |
| OpenAlex: | W3097403934 |